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MSCI 660 Liberty University Principles of Cellular Biology Exam 2

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Exam 2
Liberty University
Principles of Cellular Biology
MSCI 660
BIOM 615
Question 1
2.5 out of 2.5 points
Indicate whether the location of each of the following is topologically equivalent to the cytosol (C) or the
extracellular space (E). Your answer would be a five-letter string composed of letters C and E only, e.g.
CCECE.
( ) Ribosomes
( ) Chromatin
( ) Lysosomal hydrolases
( ) Calcium ions in the ER
( ) Peroxisomal catalase
Selected Answer:
CCEEE
Question 2
2.5 out of 2.5 points
The multidrug resistance protein is a member of this family.
Selected Answer:
an ABC transporter
Question 3
2.5 out of 2.5 points
In the following schematic diagram of a nuclear pore complex, on which side is the cytosol located? What
is the approximate diameter of the pore, as indicated by the vertical bar?
Selected Answer:
Left; 0.1 µm
Question 4
2.5 out of 2.5 points
According to the model for nuclear transport described in this chapter, what do you think would happen if
you could artificially limit all Ran-GAP activity to the nucleus and all Ran-GEF activity to the cytosol?
Proteins containing an NLS would be actively exported from the nucleus, while NES-
containing proteins would be actively imported.
Question 5
2.5 out of 2.5 points
Indicate true (T) and false (F) statements below regarding the mitochondrial protein import system. Your
answer would be a four-letter string composed of letters T and F only, e.g. TTTF.
( ) Mitochondrial proteins should fold natively twice: once in the cytosol and once inside the organelle.

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( ) β-barrel proteins that are abundant in the mitochondrial outer membrane are imported from the
cytosol independently of the TOM complex.
( ) Signal sequences that target precursor proteins to the mitochondrial matrix form an α- helix in which
positively charged residues cluster near its N-terminus, while uncharged or hydrophobic residues cluster
near the other side.
( ) At least two signal sequences are required to direct proteins to the mitochondrial matrix.
Selected Answer:
FFFF
Question 6
2.5 out of 2.5 points
Misfolded proteins in the ER may actively undergo any of the following EXCEPT ...
Selected Answer:
being expelled to the Golgi apparatus for disposal.
Question 7
2.5 out of 2.5 points
Consider two cells, A and B. Both are approximately spherical, but cell A is a bacterium with a diameter of
only about 1 µm, while the diameter of the eukaryotic cell B is about 10 µm. If the plasma membrane in
the eukaryotic cell comprises only about 2% of the total cell membrane, which cell has a higher ratio of
total cell membrane to volume?
Selected Answer:
B
Question 8
2.5 out of 2.5 points
The pumps in this family are responsible for the acidification of synaptic vesicles.
Selected Answer:
a V-type pump
Question 9
2.5 out of 2.5 points
Aquaporin has a pair of key asparagine residues located on the wall almost halfway through its pore.
These residues simultaneously bind to the oxygen atom of a passing water molecule, rendering it
unavailable for hydrogen-bonding. As a result, …
Selected Answer:
the channel cannot conduct protons.
Question 10
2.5 out of 2.5 points
The formation of a stable ternary complex involving Ran GTPase, a nuclear transport receptor, and a
cargo protein occurs in ...
Selected Answer:
nuclear export, but not import.
Question 11
2.5 out of 2.5 points
Consider a human cell such as a hepatocyte. Which of the following compartments occupies a larger
volume in the cell?

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Exam 2 Liberty University Principles of Cellular Biology MSCI 660 BIOM 615 • Question 1 2.5 out of 2.5 points Indicate whether the location of each of the following is topologically equivalent to the cytosol (C) or the extracellular space (E). Your answer would be a five-letter string composed of letters C and E only, e.g. CCECE. ( ) Ribosomes ( ) Chromatin ( ) Lysosomal hydrolases ( ) Calcium ions in the ER ( ) Peroxisomal catalase Selected Answer: CCEEE • Question 2 2.5 out of 2.5 points The multidrug resistance protein is a member of this family. Selected Answer: an ABC transporter • Question 3 2.5 out of 2.5 points In the following schematic diagram of a nuclear pore complex, on which side is the cytosol located? What is the approximate diameter of the pore, as indicated by the vertical bar? Selected Answer: Left; 0.1 µm • Question 4 2.5 out of 2.5 points According to the model for nuclear transport described in this chapter, what do you think would happen if you could artificially limit all Ran-GAP activity to the nucleus and all Ran-GEF activity to the cytosol? Selected Answer: • Question 5 Proteins containing an NLS would be actively exported from the nucleus, while NEScontaining proteins would be actively imported. 2.5 out of 2.5 points Indicate true (T) and false (F) statements below regarding the mitochondrial protein import system. Your answer would be a four-letter string composed of letters T and F only, e.g. TTTF. ( ) Mitochondrial ...
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