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San Diego City College

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Detection of SARS Coronavirus 1

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Detection of SARS Coronavirus 2

Summary

For the detection of SARS Coronavirus, it is crucial to develop the best technique of choice that

can be used to detect the virus. Since living cells have generic materials in the form of Ribose

nuclear acids (RNA) and De-ox ribose nuclei acids (DNA), it is easy to detect any virus. DNA is

found in all living cells, not the case for RNA, but Ribose nuclear acids do different functions as

DNA. For the case of Coronavirus, diagnosis of the infection is made within 8-10 hours; thus,

there is a high accuracy of results. PCR provides ways of making several copies of DNA.

Primers can be RNA OR DNA, but in PCR, DNA primers are typically used. PCR sequence

involves denaturing of DNA, annealing, and finally, use of DNA synthesis. Since Coronavirus

uses RNA material instead of DNA, there is a need to convert the RNA to DNA. The virus uses

real-time reverse transcription, which is a PCR test. RNA is converted using the reverse

transcription process to get complimentary DNA (cDNA). (Tong et al., 2020)Several copies of

CDNA are made in the PCR test to detect the virus. PCR makes billions of copies of DNA

samples. This helps scientists to take a copy of DNA and help amplify it to a large amount to

study it.

For the case of reverse transcriptase, we get that Coronavirus contains the only RNA. Once in the

cell of a living organism, the virus uses RNA to control the cell and make it into a virus-making

factory. The effect of detection of DNA since in traditional PCR, only DNA can be detected.

Therefore, there is a need to convert RNA to DNA through a process called Reverse

Transcription. PCR-polymerase chain reaction is used through the process of reverse

transcriptase (RT-PCR). Real-time RT-PCR through the extraction of samples from an

individual, for instance, nose all proteins and fats from the sample are removed. Extracted RNA

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Detection of SARS Coronavirus Student name Course number and name Instructor’s name Due date 1 Detection of SARS Coronavirus 2 Summary For the detection of SARS Coronavirus, it is crucial to develop the best technique of choice that can be used to detect the virus. Since living cells have generic materials in the form of Ribose nuclear acids (RNA) and De-ox ribose nuclei acids (DNA), it is easy to detect any virus. DNA is found in all living cells, not the case for RNA, but Ribose nuclear acids do different functions as DNA. For the case of Coronavirus, diagnosis of the infection is made within 8-10 hours; thus, there is a high accuracy of results. PCR provides ways of making several copies of DNA. Primers can be RNA OR DNA, but in PCR, DNA primers are typically used. PCR sequence involves denaturing of DNA, annealing, and finally, use of DNA synthesis. Since Coronavirus uses RNA material instead of DNA, there is a need to convert the RNA to DNA. The virus uses real-time reverse transcription, which is a PCR test. RNA is converted using the reverse transcription process to get complimentary DNA (cDNA). (Tong et al., 2020)Several copies of CDNA are made in the PCR test to de ...
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