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Biology Exercise

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Biology
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Biology exercise
Name:
Course:
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Spectrophotometer
1. What is a cuvette?
A cuvette is a liquid sample holder that resembles a small tube-like container with visible sides.
Cuvettes are used to take absorbance readings.
2. Why are fingerprints on the cuvette a problem?
Fingerprints are a problem because they affect the concentration readings. Furthermore,
concentration increases since the solution absorb more light, leading to a reduction in the
absorbance analysis (BioNetwork, 2012).
3. Why is a control (blank) needed when using a spectrophotometer?
Using a blank allows you to change the instrument to avoid any light absorbed by the solution
and examine the one absorbed by the chromophore.
4. What does "nm" mean?
Nm means Nanometer/nanometers.
5. Why is it important to position the cuvette in the correct orientation?
It is crucial to position the cuvette in the correct direction to avoid getting significantly different
readings.
6. Why is it important to close the lid of the chamber after the cuvette is in position?
It is crucial to immediately close the chamber lid after placing the cuvette to avoid absorbing
light from the outside.
7. If you have 10 samples, do you have to reset on the blank between each sample?
Yes. This is crucial to avoid adding the absorption measurement of cuvette and water to the dye
measurement. Besides, the aim is to examine the dye measurement and not the water and cuvette
included.

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1 Biology exercise Name: Course: Professor: Date: 2 Spectrophotometer 1. What is a cuvette? A cuvette is a liquid sample holder that resembles a small tube-like container with visible sides. Cuvettes are used to take absorbance readings. 2. Why are fingerprints on the cuvette a problem? Fingerprints are a problem because they affect the concentration readings. Furthermore, concentration increases since the solution absorb more light, leading to a reduction in the absorbance analysis (BioNetwork, 2012). 3. Why is a control (blank) needed when using a spectrophotometer? Using a blank allows you to change the instrument to avoid any light absorbed by the solution and examine the one absorbed by the chromophore. 4. What does "nm" mean? Nm means Nanometer/nanometers. 5. Why is it important to position the cuvette in the correct orientation? It is crucial to position the cuvette in the correct direction to avoid getting significantly different readings. 6. Why is it important to close the lid of the chamber after the cuvette is in position? It is crucial to immediately close the chamber lid after placing the cuvette to avoid absorbing light from the outside. 7. If you have 10 samples, do you have to reset on the blank between each sample? Yes. This is crucial to avoid adding the absorption measurement of cuvette and water to the dye measurement. Besides, the aim is to examine the dye measurement and not the water and cuvette included. 3 8. If a sample has a high absorbance, i ...
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