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Molecular Cloning

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Biology
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University of Central Lancashire, Preston
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Running Head: MOLECULAR MEDICINE REPORT 1
MOLECULAR CLONING
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MOLECULAR MEDICINE REPORT 2
Abstract
Molecular cloning is the assembly of DNA molecules into a vector including the subsequent
organism transformation either yeast or bacteria. It is a term that comprises several techniques.
The objectives under this report include the following to prove the numerous strategies for
performing molecular cloning. The procedures for isolating the DNA include the following;
separating the DNA using gel electrophoresis, perform DNA extraction, use a restriction enzyme
and perform DNA sequencing. At the constraint site, constraint enzymes are the ones responsible
for breaking the bond between phosphodiester. Production of clones of bacteria is through fixing
the bacteria on plates. Of all the steps identified under molecular cloning nucleic acid, purification
is the most basic one (Green and Sambrook 2017). This paper covers the several methods we can
use to conduct experiment for molecular cloning and give discussion about the results given.
Several learnable techniques of molecular cloning exist in the molecular cloning lab and they
include methods of preparation for the DNA and the vector. By comparing the distance with the
molecular weight standard samples, we can estimate the size of the samples of the nucleic acid.
Introduction
Molecular cloning is vitally done by using a gene fragment. The use of PCR analysis in the
enlargement of the fragment after separation has been done. Constraint enzymes are used to break
the bond between phosphodiester at the constraint site. At this constraint type, enzymes proceed
to digest DNA to form two ends. Thereafter these ends join. To add to it, restriction endonucleases
serve to slice the plasmids, these DNA pieces serve with inhibiting enzymes. Plasmids are circular.
The transfer of genetic materials between different cells is by bacterial plasmids. The plasmids
give various types of different characteristics, which are important in molecular cloning; they are
specifically kept for their ability to exploit DNA.

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Running Head: MOLECULAR MEDICINE REPORT MOLECULAR CLONING Name Institution Affiliation Date 1 MOLECULAR MEDICINE REPORT 2 Abstract Molecular cloning is the assembly of DNA molecules into a vector including the subsequent organism transformation either yeast or bacteria. It is a term that comprises several techniques. The objectives under this report include the following to prove the numerous strategies for performing molecular cloning. The procedures for isolating the DNA include the following; separating the DNA using gel electrophoresis, perform DNA extraction, use a restriction enzyme and perform DNA sequencing. At the constraint site, constraint enzymes are the ones responsible for breaking the bond between phosphodiester. Production of clones of bacteria is through fixing the bacteria on plates. Of all the steps identified under molecular cloning nucleic acid, purification is the most basic one (Green and Sambrook 2017). This paper covers the several methods we can use to conduct experiment for molecular cloning and give discussion about the results given. Several learnable techniques of molecular cloning exist in the molecular cloning lab and they include methods of preparation for the DNA and the vector. By comparing the distance with the molecular weight standard samples, we can estimate the size of the samples of the nucleic acid. Introduction Molecular cloning is vitally done by using a gene fragment. The use of PCR analysis in the enlargement of the fragment ...
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