Students Name Instructors Name Course Name Date Fluorescence quenching Quantum yield Fluorescence quenching is a process of relaxation that decreases the fluorescence intensity of a sample. It is caused by interaction of molecules including molecular rearrangements, exited-state reactions, energy transfer and etc. Quenching occurs without any permanent change in molecules. Fluorescence life time and quantum yield are most important in study of fluorescence. Fluorescence lifetime (Ƭ) is the time spend by the fluorophore in the exited state before returning to the ground state and fluorescence quantum yield (QY) is the ration of number of emitted photons to the number of absorbed photons. It is defined by the relative rates of radiative (Kr) and nonradiative (Knr) pathways. The fluorescent Quantum yield is proportional to the fluorescent life time and the fluorescence life time depends on ...
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