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scientific poster
Each student is required to produce a poster presentation. In each poster student will choose
one advanced laboratory technique that was used in a scientific paper and summarise the
work mentioned in these papers.

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scientific poster Each student is required to produce a poster presentation. In each poster student will choose one advanced laboratory technique that was used in a scientific paper and summarise the work mentioned in these papers. Photos courtesy of the link: https://microbenotes.com/gene-cloning-requirements-principle-stepsapplications/ Brown, Terence A. Gene cloning and DNA analysis: an introduction. Wiley-blackwell, 2020. Molecular cloning Definition Molecular cloning is a series of experimental techniques utilized in molecular genetics that are applied in the assembly of recombinant DNA molecules so as to manipulate and regulation their expression within a host cell/organism. The term "cloning" here refers to the technique of replicating a single DNA molecule from a single living cell in order to produce a large population of cells with similar DNA molecules. Steps 1. Isolation of the insert Insert preparation is done by; ⎯ Polymerase chain reaction ⎯ Restriction enzyme digestion of the gene and plasmid to form compatible ends ⎯ Fractionation by agarose gel electrophoresis 2. Ligation Ligation of the insert and plasmid is done by ligase enzyme forming recombinant p ...
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