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Subject
Biochemistry
School
Florida Gulf Coast University
Type
Homework
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RESULTS
Table 1. Absorbance values for BSA standard (calibration graph).
Concentration
Absorbance
No dilution
0
0.125
1.769
0.250
0.890
0.500
1.226
0.750
1.767
1.000
0.540
1.500
1.945
2.000
2.143
Table 2. Absorbance values of milk sample 1 (milk sample name: Whole Milk)
Dilution
Absorbance
No dilution
2.3685
1:1000
0.8054
Table 3. Absorbance values of milk sample 3 (milk sample name: Almond Milk)
Dilution
Absorbance
No dilution
2.2566
1:1000
0.7900
Table 4. Absorbance values of milk sample 2 (milk sample name: Soy Milk)
Dilution
Absorbance
No dilution
2.1556
1:1000
0.8030

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Figure 1. Calibration graph
Sample calculations
Whole milk
x
1
0.8054
x
2
0.8053
x
3
0.8051
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Almond milk
x
1
0.7900

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RESULTS Table 1. Absorbance values for BSA standard (calibration graph). Concentration No dilution 0.125 0.250 0.500 0.750 1.000 1.500 2.000 Absorbance 0 1.769 0.890 1.226 1.767 0.540 1.945 2.143 Table 2. Absorbance values of milk sample 1 (milk sample name: Whole Milk) Dilution Absorbance No dilution 2.3685 1:1000 0.8054 Table 3. Absorbance values of milk sample 3 (milk sample name: Almond Milk) Dilution Absorbance No dilution 2.2566 1:1000 0.7900 Table 4. Absorbance values of milk sample 2 (milk sample name: Soy Milk) Dilution Absorbance No dilution 2.1556 1:1000 0.8030 Figure 1. Calibration graph Absorbance vs. Concentration R² = 0.3724 2.5 Absorbance A595 2 y = 0.6533x + 0.7848 1.5 1 0.5 0 0 2 4 6 8 10 Concentration (mg/ml) Sample calculations Whole milk x1 0.8054 x2 0.8053 x3 0.8051 𝑥1 − 0.7848 0.8054 − 0.7848 = = 0.0315 𝑚𝑔/𝑚𝑙 0.6533 0.6533 𝑚𝑔 𝐶𝑜𝑛𝑐𝑒𝑛𝑡𝑟𝑎𝑡𝑖𝑜𝑛 = 1000 × 100 × 0.0315 = 3.15 𝑔/100𝑚𝑔 𝑚𝑙 y1 = 𝑥2 − 0.7848 0.8052 − 0.7848 = = 0.0313 𝑚𝑔/𝑚𝑙 0.6533 0.6533 𝑚𝑔 𝐶𝑜𝑛𝑐𝑒𝑛𝑡𝑟𝑎𝑡𝑖𝑜𝑛 = 1000 × 100 × 0.0313 = 3.13 𝑔/100𝑚𝑔 𝑚𝑙 y2 = 𝑥3 − 0.7848 0.8051 − 0.7848 = = 0.0310 𝑚𝑔/𝑚𝑙 0.6533 0.6533 𝑚𝑔 𝐶𝑜𝑛𝑐𝑒𝑛𝑡𝑟𝑎𝑡𝑖𝑜𝑛 = 1000 × 100 × 0.0310 = 3.10 𝑔/100𝑚𝑔 𝑚𝑙 y3 = Almond milk x1 0.7900 x2 0.7905 x3 0.7901 𝑥1 − 0.7848 0.7900 ? ...
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