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Functional immobilization of signalin molecules to control the

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FUNCTIONAL IMMOBILIZATION OF SIGNALIN MOLECULES TO CONTROL THE FATE OF STEM CELLS Gabriela Krupskaya Villavicencio Morales 818A ◦ The mode of ligand presentation has a fundamental role in organizing cell fate throughout development. We report a rapid and simple approach for immobilizing signaling ligands to maleic anhydride copolymer thin-film coatings, enabling stable signaling ligand presentation at interfaces at defined concentrations. We demonstrate the utility of this platform technology using leukemia inhibitory factor (LIF) and stem cell factor (SCF). Immobilized LIF supported mouse embryonic stem cell (mESC) pluripotency for at least 2 weeks in the absence of added diffusible LIF. Immobilized LIF activated signal transducer and activator of transcription 3 (STAT3) and mitogenactivated protein kinase (MAPK) signaling in a dose-dependent manner. The introduced method allows for the robust investigation of cell fate responses from interfaceimmobilized ligands. ◦ The importance of exogenously added or endogenously produced soluble signal-transducing ligands in the regulation of cell fate is intensively studied. Although it is widely recognized that a substantial number of these ligands act at interfaces in vivo (that is, constrained to surfaces rather than in solution), studies on the effects of cell exposure to the non–freely diffusible forms of these molecules are just emerging. The design principles associated with bound ligand presentation are fundamental to ...
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