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Features of eukaryotic DNA
1. Which of the following is not a part of a gene?
a) Ori
b) Promoter
c) Operon
d) Terminator
2. What is the correct order for increasing gene density?
a) Bacteria, Virus, Fruit fly, Human
b) Fruit fly, Bacteria, Virus, Human
c) Human, Fruit fly, Bacteria, Virus
d) Virus, Bacteria, Fruit fly, Human
3. Which of these factors contribute to a decrease in gene density?
a) Operon
b) Intergenic sequence
c) Exon sequence
d) Cell size
5. Which of the following is not a character of unique Intergenic DNA?
a) Mutant genes
b) Pseudogenes
c) Palindromes
d) Nonfunctional gene fragments
6. Which of the following is responsible for the formation of Pseudogenes?
a) Polymerase
b) Reverse transcriptase
c) Klenow fragment
d) Wrong primer
4. What do you think is the requirement of Intergenic DNA in higher organisms?
a) Just genetic load
b) To avoid viable mutations
c) Helps in regulation of transcription
d) Helps in genome organization
5. Which of the following is not a character of unique Intergenic DNA?
a) Mutant genes
b) Pseudogenes
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c) Palindromes
d) Nonfunctional gene fragment
7. With respect to microsatellite DNA which of the following is correct?
a) Tandem repeats
b) Dinucleotide repeats
c) 100 bp units
d) Inaccurate duplicating
8. Genome wide repeat unit are generally transposable elements.
a) True
b) False
9. Repeated DNA can be referred to as junk DNA.
a) True
b) False
10. The simpler the organism the more likely they are to have transposable elements.
a) True
b) False
11 Double-stranded structure of nucleic acid is the basic requirement for palindromic
sequences.
a) True
b) False
12. Which of the following words represents a palindrome?
a) DUCK
b) MAMA
c) BOOM
d) MADAM
13. Which of the following will form a palindromic sequence?
a) ATTGCAAT
b) AGTCCTGA
c) GTTCCAAG
d) GTTGGAAC
14. Which of the following is affected by palindromic sequences?
a) Acetylation site
b) Phosphorylation site
c) Methylation site
d) Promoter sit
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15. Palindromic sequences play a very important role in gene manipulation.
a) True
b) False
16. Which of the following palindromes is not a restriction site?
a) GAATTC
b) TACGTA
c) CCTAGG
d) AGCT
17. Molecular cutters do not recognize the palindromic sequence.
a) True
b) False
8. Which of the following is an example of an inverted repeat?
a) ATTCGCGAAT
b) ATCGNNNNCGAT
c) TACCNNNNGGAT
d) AGCCNNNCCGA
19. Inverted repeat have a number of biological functions. Which of the following is a
biological function of an inverted repeat?
a) Diseases
b) Central dogma
c) Cellular metabolism
d) Genetic stability
20. Most commonly known hairpin structures are found in _____________
a) DNA
b) mRNA
c) tRNA
d) Rrna
21. Which of the following does not promote stability in stem-loop structure?
a) Length of the stem
b) Size of the loop
c) A : U base pairing
d) π orbital of aromatic ring
22. What is the minimum number of bases required for loop stability?
a) 2
3 b)
c) 4
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23. Which of the following does not contain a stem-loop structure?
a) tRNA
b) Pseudoknot
c) Ribozyme
d) rRNA
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24. Which of the following cannot be used for the separation of nucleic acids?
a) SDS PAGE
b) PAGE
c) Northern blotting
d) PAGE
The polymerization of the gel used in PAGE occurs between polyacrylamide and
____________
a) N, N acrylamide
b) Bisacrylamide
c) N methyleneacrylamide
d) N, N methylene bisacrylamide
26 If DNA is digested by endonucleases in four sites giving rise to fragments of which two
are equal in length how many bands would be seen after electrophoresis?
a) 3
b) 4
c) 5
d) 6
27 The fluorescent dye such Ethidium is used for visualizing DNA. How do ethidium binds to
DNA?
a) Stacked between histone molecules
b) Binds to the nucleotide base
c) Intercalated between the stacked bases
d) Binds to the phosphodiester backbone
28.Pulse field gel electrophoresis separates DNA molecules of size ___________
a) 10 20 bp
c) 30 50 Kb
d) 40 50 bp
Which of the following will migrate faster? The condition is the molecular weight of the
following is equal.
a) Supercoiled circular DNA
b) Nicked circular DNA
c) Single stranded DNA
d) Double stranded DNA
Agarose can be extracted from which of the following?
a) Gracilaria esculentus
b) Lycazusican esculentum
c) Ficum benghalensis
d) Agrostis stolonifera
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Electrophoresis cannot be used to separate _______________
a) DNA
b) RNA
c) Amino acid
d) Protein
Which of the following is not a character of polyacrylamide gel?
a) Inert
b) Ionic strength
c) Stable over a wide range of pH
d) Separate upto a few 100 bp of DNA
Pulse field gel electrophoresis was developed by ____________
a) Collins and John
b) Kary Mullis
c) Patrick O’ Farrell
d) Schwartz and Cantor
What is the condition required to observe B form of DNA?
a) Low humidity
b) High humidity
c) Low temperature
d) High temperature
What is the condition required to observe A form of DNA?
a) Low humidity
b) High humidity
c) Low temperature
d) High temperature
How many bases are there in per turn of the B form of DNA?
a) 8 bp
b) 9 bp
c) 10 bp
d) 1Which of the following is not a character of A DNA?
a) Short and broad
b) Left handed helix
c) Narrow minor groove
d) Broad and shallow major groove1 bp
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. How many bases are there in per turn of the A form of DNA?
a) 11 bp
b) 10 bp
c) 12 bp
d) 8 bp
Which of the following is not a character of B form of DNA?
a) Longer and thinner
b) Right-handed helix
c) Narrow minor groove
d) Flat major groove
In a right handed DNA the glycosidic bond is in syn configuration.
a) True
b) False
What is the factor responsible for the left-handed helical conformation in Z form of DNA?
a) Syn conformation at pyrimidine
b) Anti conformation at pyrimidine
c) Syn conformation at purine
d) Anti conformation at purine
In the Z form of DNA the fundamental repeating unit is __________
a) Purine pyrimidine nucleotide
b) Purine pyrimidine dinucleotide
c) Purine pyrimidine tri-nucleotide
d) Purine pyrimidine tetra-nucleotide
The two strands of cccDNA can be easily separated without any breakage of bonds.
a) True
b) False
Passing of one strand through the other in order to get separated is known as _______
a) Twist
b) Linking number
c) Writhing number
d) Toroid
inking number is always a ___________
a) Whole number
b) Prime number
c) Integer
d) Even number
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inking number is the sum of which geometric components?
a) Twist and writhe
b) Toroid and writhe
c) Twist and wrap
d) Twist and crossover
3 dimension structure of cccDNA is ___________
a) Nicked
b) Supercoiled
c) Flat
d) Torsionally stressed
Plectonomic writhe is __________
a) Cylindrical axis winding
b) Long axis twisted around itself
c) Helical turns of one strand about the other
d) Number of encounters of the two strands of a helix
Interwound writhe and spiral writhe are topologically different.
a) True
b) False
Twist and writhe are interconvertible.
a) True
b) False
. How can we remove supercoils from cccDNA?
a) Treat with DNase
b) Treat with detergent
c) Treat with endonuclease
d) Application of heat
What is the relation between linking number (Lk), writing number (Wr) and twist number
(Tw)?
a) Lk = Tw Wr
b) Lk = Wr Tw
c) Tw = Lk Wr
d) Tw = Lk + Wr
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How is the extent of supercoiling measured?
a) Lk + Lk
0
b) Lk
0
Lk
c) Lk Lk
0
d) Lk + Lk
1
. If Lk < Lk
0
then DNA is ____________
a) Relaxed
b) Negatively coiled
c) Positively coiled
d) ΔLk > 0
What is ΔLk and Lk
0
dependent upon?
a) Histone concentration
b) G : A ratio
c) Composition of DNA
d) Length of DNA
trand separation is easily accomplished in positively supercoiled DNA.
a) True
b) False
Which of the following organism has been found to have a positively supercoiled DNA?
a) Saurida tumbil
b) Monarda didyma
) Thermus aquaticus
d) Cyanobacteria
Which of the following is not true about positive supercoils?
a) Helps to avoid denaturing
b) Over wounded DNA
c) Strand separations require low energy
d) Rare form of DNA supercoiling
Packaging of DNA around nucleosome is known as ____________
a) Toroid writhes
b) Twist
c) Plectonemic writhes
d) Supercoils
Showing Page:
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Which of the following is known to relax supercoiled DNA?
a) Nuclease
b) DNase I
c) Endonuclease
d) Topoisomerase
With respect to topoisomerases which of the following is true?
a) Only of one type
b) Never changes linking number
c) Requires ATP.
Nucleosome was first described in 1974 by ___________
a) William Asbury
b) Rosalind Franklin
c) Roger Kornberg
d) John Crick
d) Introduces knots
The extent of chromosome coiling in non dividing cells is _________
a) Supercoiled
b) Euchromatin
c) Condensed
d) Heterochromatin
Why are chromosomes condensed?
a) To facilitate accommodation
b) Always condensed
c) To facilitate cell division
d) To facilitate distribution in daughter cells
At which phase do transcription ceases?
a) Interphase
b) Prophase
c) S phase
d) G phase
The part that plays a critical role in even distribution of parental DNA during division is
___________
a) Telomere
b) Centromere
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c) Spindle fibre
d) Centrioles
With respect to centromere which of the following is wrong?
a) Constricted chromosomal region
b) Holds the sister chromatids together
c) Attaches to spindle fibres
d) Facilitates even distribution
Centromeric DNA was initially defined in ___________
a) Bacteria
b) Fungi
c) Yeast
d) Human
. Telomere is not related to __________
a) Maintenance
b) Chromosome degradation
c) Division
d) Replication
. In cancer telomerase activity __________
a) Increases
b) Decreases
c) Remains constant
d) Plays no role

Unformatted Attachment Preview

Features of eukaryotic DNA 1. Which of the following is not a part of a gene? a) Ori b) Promoter c) Operon d) Terminator 2. What is the correct order for increasing gene density? a) Bacteria, Virus, Fruit fly, Human b) Fruit fly, Bacteria, Virus, Human c) Human, Fruit fly, Bacteria, Virus d) Virus, Bacteria, Fruit fly, Human 3. Which of these factors contribute to a decrease in gene density? a) Operon b) Intergenic sequence c) Exon sequence d) Cell size 5. Which of the following is not a character of unique Intergenic DNA? a) Mutant genes b) Pseudogenes c) Palindromes d) Nonfunctional gene fragments 6. Which of the following is responsible for the formation of Pseudogenes? a) Polymerase b) Reverse transcriptase c) Klenow fragment d) Wrong primer 4. What do you think is the requirement of Intergenic DNA in higher organisms? a) Just genetic load b) To avoid viable mutations c) Helps in regulation of transcription d) Helps in genome organization 5. Which of the following is not a character of unique Intergenic DNA? a) Mutant genes b) Pseudogenes c) Palindromes d) Nonfunctional gene fragment 7. With respect to microsatellite DNA which of the following is correct? a) Tandem repeats b) Dinucleotide repeats c) 100 bp units d) Inaccurate duplicating 8. Genome – wide repeat unit are generally transposable elements. a) True b) False 9. Repeated DNA can be referred to as junk DNA. a) True b) False 10. The simpler the organism the more likely they are to have transposable elements. a) True b) False 11 Double-stranded structure of nucleic acid is the basic requirement for palindromic sequences. a) True b) False 12. Which of the following words represents a palindrome? a) DUCK b) MAMA c) BOOM d) MADAM 13. Which of the following will form a palindromic sequence? a) ATTGCAAT b) AGTCCTGA c) GTTCCAAG d) GTTGGAAC 14. Which of the following is affected by palindromic sequences? a) Acetylation site b) Phosphorylation site c) Methylation site d) Promoter sit 15. Palindromic sequences play a very important role in gene manipulation. a) True b) False 16. Which of the following palindromes is not a restriction site? a) GAATTC b) TACGTA c) CCTAGG d) AGCT 17. Molecular cutters do not recognize the palindromic sequence. a) True b) False 8. Which of the following is an example of an inverted repeat? a) ATTCGCGAAT b) ATCGNNNNCGAT c) TACCNNNNGGAT d) AGCCNNNCCGA 19. Inverted repeat have a number of biological functions. Which of the following is a biological function of an inverted repeat? a) Diseases b) Central dogma c) Cellular metabolism d) Genetic stability 20. Most commonly known hairpin structures are found in _____________ a) DNA b) mRNA c) tRNA d) Rrna 21. Which of the following does not promote stability in stem-loop structure? a) Length of the stem b) Size of the loop c) A : U base pairing d) π orbital of aromatic ring 22. What is the minimum number of bases required for loop stability? a) 2 3 b) c) 4 23. Which of the following does not contain a stem-loop structure? a) tRNA b) Pseudoknot c) Ribozyme d) rRNA 24. Which of the following cannot be used for the separation of nucleic acids? a) SDS – PAGE b) PAGE c) Northern blotting d) PAGE The polymerization of the gel used in PAGE occurs between polyacrylamide and ____________ a) N, N – acrylamide b) Bisacrylamide c) N – methyleneacrylamide d) N, N – methylene bisacrylamide 26 If DNA is digested by endonucleases in four sites giving rise to fragments of which two are equal in length how many bands would be seen after electrophoresis? a) 3 b) 4 c) 5 d) 6 27 The fluorescent dye such Ethidium is used for visualizing DNA. How do ethidium binds to DNA? a) Stacked between histone molecules b) Binds to the nucleotide base c) Intercalated between the stacked bases d) Binds to the phosphodiester backbone 28.Pulse field gel electrophoresis separates DNA molecules of size ___________ a) 10 – 20 bp c) 30 – 50 Kb d) 40 – 50 bp Which of the following will migrate faster? The condition is the molecular weight of the following is equal. a) Supercoiled circular DNA b) Nicked circular DNA c) Single stranded DNA d) Double stranded DNA Agarose can be extracted from which of the following? a) Gracilaria esculentus b) Lycazusican esculentum c) Ficum benghalensis d) Agrostis stolonifera Electrophoresis cannot be used to separate _______________ a) DNA b) RNA c) Amino acid d) Protein Which of the following is not a character of polyacrylamide gel? a) Inert b) Ionic strength c) Stable over a wide range of pH d) Separate upto a few 100 bp of DNA Pulse field gel electrophoresis was developed by ____________ a) Collins and John b) Kary Mullis c) Patrick O’ Farrell d) Schwartz and Cantor What is the condition required to observe B form of DNA? a) Low humidity b) High humidity c) Low temperature d) High temperature What is the condition required to observe A form of DNA? a) Low humidity b) High humidity c) Low temperature d) High temperature How many bases are there in per turn of the B form of DNA? a) 8 bp b) 9 bp c) 10 bp d) 1Which of the following is not a character of A DNA? a) Short and broad b) Left handed helix c) Narrow minor groove d) Broad and shallow major groove1 bp . How many bases are there in per turn of the A form of DNA? a) 11 bp b) 10 bp c) 12 bp d) 8 bp Which of the following is not a character of B form of DNA? a) Longer and thinner b) Right-handed helix c) Narrow minor groove d) Flat major groove In a right handed DNA the glycosidic bond is in syn configuration. a) True b) False What is the factor responsible for the left-handed helical conformation in Z form of DNA? a) Syn conformation at pyrimidine b) Anti conformation at pyrimidine c) Syn conformation at purine d) Anti conformation at purine In the Z form of DNA the fundamental repeating unit is __________ a) Purine – pyrimidine nucleotide b) Purine – pyrimidine dinucleotide c) Purine – pyrimidine tri-nucleotide d) Purine – pyrimidine tetra-nucleotide The two strands of cccDNA can be easily separated without any breakage of bonds. a) True b) False Passing of one strand through the other in order to get separated is known as _______ a) Twist b) Linking number c) Writhing number d) Toroid inking number is always a ___________ a) Whole number b) Prime number c) Integer d) Even number inking number is the sum of which geometric components? a) Twist and writhe b) Toroid and writhe c) Twist and wrap d) Twist and crossover 3– dimension structure of cccDNA is ___________ a) Nicked b) Supercoiled c) Flat d) Torsionally stressed Plectonomic writhe is __________ a) Cylindrical axis winding b) Long axis twisted around itself c) Helical turns of one strand about the other d) Number of encounters of the two strands of a helix Interwound writhe and spiral writhe are topologically different. a) True b) False Twist and writhe are interconvertible. a) True b) False . How can we remove supercoils from cccDNA? a) Treat with DNase b) Treat with detergent c) Treat with endonuclease d) Application of heat What is the relation between linking number (Lk), writing number (Wr) and twist number (Tw)? a) Lk = Tw – Wr b) Lk = Wr – Tw c) Tw = Lk – Wr d) Tw = Lk + Wr How is the extent of supercoiling measured? a) Lk + Lk0 b) Lk0 – Lk c) Lk – Lk0 d) Lk + Lk1 . If Lk < Lk0 then DNA is ____________ a) Relaxed b) Negatively coiled c) Positively coiled d) ΔLk > 0 What is ΔLk and Lk0 dependent upon? a) Histone concentration b) G : A ratio c) Composition of DNA d) Length of DNA trand separation is easily accomplished in positively supercoiled DNA. a) True b) False Which of the following organism has been found to have a positively supercoiled DNA? a) Saurida tumbil b) Monarda didyma ) Thermus aquaticus d) Cyanobacteria Which of the following is not true about positive supercoils? a) Helps to avoid denaturing b) Over wounded DNA c) Strand separations require low energy d) Rare form of DNA supercoiling Packaging of DNA around nucleosome is known as ____________ a) Toroid writhes b) Twist c) Plectonemic writhes d) Supercoils Which of the following is known to relax supercoiled DNA? a) Nuclease b) DNase I c) Endonuclease d) Topoisomerase With respect to topoisomerases which of the following is true? a) Only of one type b) Never changes linking number c) Requires ATP. Nucleosome was first described in 1974 by ___________ a) William Asbury b) Rosalind Franklin c) Roger Kornberg d) John Crick d) Introduces knots The extent of chromosome coiling in non – dividing cells is _________ a) Supercoiled b) Euchromatin c) Condensed d) Heterochromatin Why are chromosomes condensed? a) To facilitate accommodation b) Always condensed c) To facilitate cell division d) To facilitate distribution in daughter cells At which phase do transcription ceases? a) Interphase b) Prophase c) S phase d) G phase The part that plays a critical role in even distribution of parental DNA during division is ___________ a) Telomere b) Centromere c) Spindle fibre d) Centrioles With respect to centromere which of the following is wrong? a) Constricted chromosomal region b) Holds the sister chromatids together c) Attaches to spindle fibres d) Facilitates even distribution Centromeric DNA was initially defined in ___________ a) Bacteria b) Fungi c) Yeast d) Human . Telomere is not related to __________ a) Maintenance b) Chromosome degradation c) Division d) Replication . In cancer telomerase activity __________ a) Increases b) Decreases c) Remains constant d) Plays no role Name: Description: ...
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