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What Is a Refractometer?
A refractometer is a device which measures the content (brix or sugar, honey, urine, salt, battery
fluid…) of a solution by the refraction of light. Using an optical refractometer is simply a matter
of placing several drops of the sample liquid on the angled prism and sealing the clear plate on
top of it. Look through the eyepiece while pointing the refractometer at a source of direct light.
The use of a digital refractometer eliminates the uncertainty associated with optical
refractometers; a digital refractometer uses an LED light to shine through the sample. When a
liquid sample is applied to the measuring surface only some of the light is transmitted through
the solution and is lost; while the remaining light is reflected onto a linear array of photodiodes
creating a shadow line. The refractive index is directly related to the position of the shadow line
on the photodiodes. Once the position of the shadow line has been automatically determined by
the instrument, the internal software will correlate the position to refractive index, or to another
unit of measure related to refractive index, and display a digital readout.
One benefit to a refractometer is that most models have an automatic temperature conversion, so
samples can be taken directly from a production line. The second benefit is that only a very small
sample is needed. A couple drops of liquid will give an accurate reading.
What does a refractometer measure?
A refractometer is used to determine a concentration of a particular substance within a given
solution. It operates based on the principle of refraction. When rays of light pass from one
medium into another, they are bent either toward or away from a normal line between the two
media.
Working principle:
Refractometers measure the degree to which the light changes direction, called the angle of
refraction. A refractometer takes the refraction angles and correlates them to refractive index
(nD) values that have been established. ... Measurements are read at the point where the prism
and solution meet.
Using a Refractometer for Sugar Analysis
1. Place deionized or distilled water on the sample well. ...
2. Press the Zero button.
3. Remove the water from the prism.
4. Place your sample on the sample well.
5. Take the refractometer reading.
6. Clean the prism and repeat for any other measurements.
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How do you take care of a refractometer?
To clean the refractometer use a soft cloth or soft tissue paper moistened with water and wipe
the prism and bottom surfaces of the cover plate. Dry the surfaces with a soft cloth or tissue
paper. If the surfaces are not well cleaned before the next sample is loaded, an erroneous or
fuzzy dividing line may result.
How much sugar is in a Brix?
Brix is a measure of the amount of dissolved solids in a liquid via its specific gravity, and is
used especially to measure dissolved sugar. One degree Brix is 1 gram of sucrose in 100 grams
of solution.
Do refractometers need calibration?
Refractometers should be calibrated at the beginning of each use and, depending on how many
samples are being measured, periodically throughout the sampling process. Proper calibration
requires a pure water source and testing solutions of known sucrose concentration.

Unformatted Attachment Preview

What Is a Refractometer? A refractometer is a device which measures the content (brix or sugar, honey, urine, salt, battery fluid…) of a solution by the refraction of light. Using an optical refractometer is simply a matter of placing several drops of the sample liquid on the angled prism and sealing the clear plate on top of it. Look through the eyepiece while pointing the refractometer at a source of direct light. The use of a digital refractometer eliminates the uncertainty associated with optical refractometers; a digital refractometer uses an LED light to shine through the sample. When a liquid sample is applied to the measuring surface only some of the light is transmitted through the solution and is lost; while the remaining light is reflected onto a linear array of photodiodes creating a shadow line. The refractive index is directly related to the position of the shadow line on the photodiodes. Once the position of the shadow line has been automatically determined by the instrument, the internal software will correlate the position to refractive index, or to another unit of measure related to refractive index, and display a digital readout. One benefit to a refractometer is that most models have an automatic temperature conversion, so samples can be taken directly from a production line. The second benefit is that only a very small sample is needed. A couple drops of liquid will give an accurate reading. What does a refractometer measure? A refractometer is used to determine a concentration of a particular substance within a given solution. It operates based on the principle of refraction. When rays of light pass from one medium into another, they are bent either toward or away from a normal line between the two media. Working principle: Refractometers measure the degree to which the light changes direction, called the angle of refraction. A refractometer takes the refraction angles and correlates them to refractive index (nD) values that have been established. ... Measurements are read at the point where the prism and solution meet. Using a Refractometer for Sugar Analysis 1. 2. 3. 4. 5. 6. Place deionized or distilled water on the sample well. ... Press the Zero button. Remove the water from the prism. Place your sample on the sample well. Take the refractometer reading. Clean the prism and repeat for any other measurements. How do you take care of a refractometer? To clean the refractometer use a soft cloth or soft tissue paper moistened with water and wipe the prism and bottom surfaces of the cover plate. Dry the surfaces with a soft cloth or tissue paper. If the surfaces are not well cleaned before the next sample is loaded, an erroneous or fuzzy dividing line may result. How much sugar is in a Brix? Brix is a measure of the amount of dissolved solids in a liquid via its specific gravity, and is used especially to measure dissolved sugar. One degree Brix is 1 gram of sucrose in 100 grams of solution. Do refractometers need calibration? Refractometers should be calibrated at the beginning of each use and, depending on how many samples are being measured, periodically throughout the sampling process. Proper calibration requires a pure water source and testing solutions of known sucrose concentration. Name: Description: ...
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