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
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/
C1


ELISA


17/1/2022
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What is an ELISA test?
An enzyme-linked immunosorbent assay, also
called ELISA or EIA, is a test that detects and
measures antibodies in your blood. This test can be
used to determine if you have antibodies related to
certain infectious conditions. Antibodies are proteins
that your body produces in response to harmful
substances called antigens.
An ELISA test may be used to diagnose:
HIV, which causes AIDS
1- Lyme disease
2- pernicious anemia
3- Rocky Mountain spotted fever
4- rotavirus
5- squamous cell carcinoma
6- syphilis
7- toxoplasmosis
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How is the test performed ?
The ELISA test involves taking a sample of your
blood. First, a healthcare provider will cleanse your
arm with an antiseptic. Then, a tourniquet, or band,
will be applied around your arm to create pressure
and cause your veins to swell with blood. Next, a
needle will be placed in one of your veins to draw a
small sample of blood. When enough blood has been
collected, the needle will be removed and a small
bandage will be placed on your arm where the needle
was. You’ll be asked to maintain pressure at the site
where the needle was inserted for a few minutes to
reduce blood flow
The blood sample will be sent to a laboratory for
analysis. In the lab, a technician will add the sample
to a petri dish containing the specific antigen related
to the condition for which you are being tested. If
your blood contains antibodies to the antigen, the two
will bind together. The technician will check this by
adding an enzyme to the petri dish and observing how
your blood and the antigen react.
How the test results are reported varies based on the
laboratory that conducts the analysis. It also depends
on the condition for which you’re being tested. Your
doctor should discuss your results and what they
mean. Sometimes, a positive result will mean that you
don’t have the condition.

Unformatted Attachment Preview

‫كلية الحدباء الجامعة‬ ‫االســـــم‪ :‬معاذ محمد يحيى‬ ‫المرحلى‪ :‬الثالثة ‪/‬صباحي‬ ‫الشعبــة‪C1 :‬‬ ‫المــادة‪:‬تقنيات مختبرية متقدمة‬ ‫تقرير عن‪:‬‬ ‫(‪)ELISA‬‬ ‫مدرس المادة‬ ‫م‪.‬م حسن خالد محمد طاهر‬ ‫تاريخ تسليم التقرير‪ :‬السبت‪17/1/2022‬‬ What is an ELISA test? An enzyme-linked immunosorbent assay, also called ELISA or EIA, is a test that detects and measures antibodies in your blood. This test can be used to determine if you have antibodies related to certain infectious conditions. Antibodies are proteins that your body produces in response to harmful substances called antigens. An ELISA test may be used to diagnose: HIV, which causes AIDS 1- Lyme disease 2- pernicious anemia 3- Rocky Mountain spotted fever 4- rotavirus 5- squamous cell carcinoma 6- syphilis 7- toxoplasmosis How is the test performed ? The ELISA test involves taking a sample of your blood. First, a healthcare provider will cleanse your arm with an antiseptic. Then, a tourniquet, or band, will be applied around your arm to create pressure and cause your veins to swell with blood. Next, a needle will be placed in one of your veins to draw a small sample of blood. When enough blood has been collected, the needle will be removed and a small bandage will be placed on your arm where the needle was. You’ll be asked to maintain pressure at the site where the needle was inserted for a few minutes to reduce blood flow. The blood sample will be sent to a laboratory for analysis. In the lab, a technician will add the sample to a petri dish containing the specific antigen related to the condition for which you are being tested. If your blood contains antibodies to the antigen, the two will bind together. The technician will check this by adding an enzyme to the petri dish and observing how your blood and the antigen react. How the test results are reported varies based on the laboratory that conducts the analysis. It also depends on the condition for which you’re being tested. Your doctor should discuss your results and what they mean. Sometimes, a positive result will mean that you don’t have the condition. Name: Description: ...
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