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Recombinant DNA UCSD Crisper Report Essay
Hello, I would like you to follow the steps below as carful as you can. I have attached sample of the lab report that I wa ...
Recombinant DNA UCSD Crisper Report Essay
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The aim of this activity is to reinforce the ethical approach to the process of science, as well as to introduce the ethical standards applied when animals are used in research.Watch two short documents 1. Genes as Medicine (Links to an external site.).2. Blue Baby Syndrome (Links to an external site.)These movies provide you an authentic example of the process of science and how animal models are chosen for scientific research to develop a gene therapy for a congenital form of blindness.Provide answers to the following questions (Links to an external site.) Answer questions 1-8 only Skip question 9 and 10.
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Lab 6 Part B. Restriction Site Mapping of a Polymorphic Mitochondrial DNA (mtDNA) Mitochondrial DNA is DNA that is located ...
just answer section 3
Lab 6 Part B. Restriction Site Mapping of a Polymorphic Mitochondrial DNA (mtDNA) Mitochondrial DNA is DNA that is located in the mitochondria, organelle that converts chemical
energy from food into ATP. MtDNA is a small portion of DNA found in cells, but since this
evolves faster than nuclear DNA it has become important in phylogenetics and evolutionary
biology. This DNA is inherited from the mother. This DNA is highly conserved and has a slow
mutation rate, but polymorphisms can occur. A polymorphism is the presence of genetic
variation within a population, upon which natural selection can occur. Today students will map a
polymorphic mtDNA.
Section 1: You have isolated some mitochondrial DNA (mtDNA) from Walleye (Sander vitreous).
The mtDNA was digested with four restriction endonucleases Apa Ⅰ, Bcl Ⅱ, Cla Ⅰ, and Dra
Ⅰ. The fragments obtained (sizes in kilobase pairs) with each enzyme were as follows: Apa Ⅰ 11.5, 3.0, 2.0
Bcl Ⅱ 1 cut only – fragment size 16.5 Cla Ⅰ 11.3, 5.2 Dra Ⅰ 13.0, 3.5 You wish to map these restriction sites on the molecule, so you conduct a series of double
digests, which give the following sized fragments:
Apa I/Bcl II 10.0, 3.0, 2.0, 1.5 Apa I/Cla Ⅰ 7.5, 4.0, 2.0, 1.8, 1.2 Apa I/Dra I 9.5, 3.0, 2.0, 1.5, 0.5 Bcl II/Cla I 6.0, 5.3, 5.2 Bcl II/Dra I 13.0, 3.0, 0.5 Cla I/Dra I 5.5, 5.2, 3.5, 2.3 Draw a linear restriction site map for this walleye mtDNA that is opened at the single Bcl Ⅱ
restriction site. Show all maps neatly. Section 2: A polymorphism is now found using Dra Ⅰ such that the new fragment pattern for this
enzyme becomes: 8.0, 5.0, 3.5. What has happened?
You conduct two more double digests in order to map this variable site, giving results as
follows: Cla I/Dra I 5.2, 5.0, 3.5, 2.3, 0.5 Apa I/Dra I 5.0, 4.5, 3.0, 2.0, 1.5, 0.5
Draw a map of these new double digests and show the variable site with an *.
Draw a picture of a gel showing all fragment samples used in this section. Section 3: You examine another individual with Dra Ⅰ revealing the following fragment results:
9.0, 5.0, 3.5. You think that this is a bit odd. So, you conduct a few more digests, getting the
following results: Apa Ⅰ 11.5, 4.0, 2.0 Cla Ⅰ 11.3, 6.2 Where is this phenomenon affecting the mtDNA molecule? Indicate the portion of the
molecule that is affected. What fragments would you expect to result from a Cla Ⅰ/Dra Ⅰ
double digest on this individual?
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Most Popular Content
Recombinant DNA UCSD Crisper Report Essay
Hello, I would like you to follow the steps below as carful as you can. I have attached sample of the lab report that I wa ...
Recombinant DNA UCSD Crisper Report Essay
Hello, I would like you to follow the steps below as carful as you can. I have attached sample of the lab report that I want you to go ahead and paraphrase it as much as possible but in your own word and the make sure that the sentences make senses. I have also attached a file for all the steps that need to be done in this assignment. In this lab try to focus on the Background and method part but not the result. Again Background and Method. please if you do not have good knolgedwge in molecular biology, genetics, and Recombinant DNA lab then do not select this question. Thank you so much!
Google Earth Oceanography Assignment
I'm looking for a word document that has the answers to the questions provided in the Oceanography document. Google Earth ...
Google Earth Oceanography Assignment
I'm looking for a word document that has the answers to the questions provided in the Oceanography document. Google Earth is required, and I have two KMZ documents needed (Google Earth files) that I can give over google drive. There's a total of 25 questions.
10 pages
Solubility Of Organic Compounds
The purpose of this experiment is to determine the extent of solubility of organic compounds in different solvents. Miscib ...
Solubility Of Organic Compounds
The purpose of this experiment is to determine the extent of solubility of organic compounds in different solvents. Miscibility of mixture of polar ...
UCSD Ethical Standards Applied in Animals Research Discussion
The aim of this activity is to reinforce the ethical approach to the process of science, as well as to introduce the ethi ...
UCSD Ethical Standards Applied in Animals Research Discussion
The aim of this activity is to reinforce the ethical approach to the process of science, as well as to introduce the ethical standards applied when animals are used in research.Watch two short documents 1. Genes as Medicine (Links to an external site.).2. Blue Baby Syndrome (Links to an external site.)These movies provide you an authentic example of the process of science and how animal models are chosen for scientific research to develop a gene therapy for a congenital form of blindness.Provide answers to the following questions (Links to an external site.) Answer questions 1-8 only Skip question 9 and 10.
just answer section 3
Lab 6 Part B. Restriction Site Mapping of a Polymorphic Mitochondrial DNA (mtDNA) Mitochondrial DNA is DNA that is located ...
just answer section 3
Lab 6 Part B. Restriction Site Mapping of a Polymorphic Mitochondrial DNA (mtDNA) Mitochondrial DNA is DNA that is located in the mitochondria, organelle that converts chemical
energy from food into ATP. MtDNA is a small portion of DNA found in cells, but since this
evolves faster than nuclear DNA it has become important in phylogenetics and evolutionary
biology. This DNA is inherited from the mother. This DNA is highly conserved and has a slow
mutation rate, but polymorphisms can occur. A polymorphism is the presence of genetic
variation within a population, upon which natural selection can occur. Today students will map a
polymorphic mtDNA.
Section 1: You have isolated some mitochondrial DNA (mtDNA) from Walleye (Sander vitreous).
The mtDNA was digested with four restriction endonucleases Apa Ⅰ, Bcl Ⅱ, Cla Ⅰ, and Dra
Ⅰ. The fragments obtained (sizes in kilobase pairs) with each enzyme were as follows: Apa Ⅰ 11.5, 3.0, 2.0
Bcl Ⅱ 1 cut only – fragment size 16.5 Cla Ⅰ 11.3, 5.2 Dra Ⅰ 13.0, 3.5 You wish to map these restriction sites on the molecule, so you conduct a series of double
digests, which give the following sized fragments:
Apa I/Bcl II 10.0, 3.0, 2.0, 1.5 Apa I/Cla Ⅰ 7.5, 4.0, 2.0, 1.8, 1.2 Apa I/Dra I 9.5, 3.0, 2.0, 1.5, 0.5 Bcl II/Cla I 6.0, 5.3, 5.2 Bcl II/Dra I 13.0, 3.0, 0.5 Cla I/Dra I 5.5, 5.2, 3.5, 2.3 Draw a linear restriction site map for this walleye mtDNA that is opened at the single Bcl Ⅱ
restriction site. Show all maps neatly. Section 2: A polymorphism is now found using Dra Ⅰ such that the new fragment pattern for this
enzyme becomes: 8.0, 5.0, 3.5. What has happened?
You conduct two more double digests in order to map this variable site, giving results as
follows: Cla I/Dra I 5.2, 5.0, 3.5, 2.3, 0.5 Apa I/Dra I 5.0, 4.5, 3.0, 2.0, 1.5, 0.5
Draw a map of these new double digests and show the variable site with an *.
Draw a picture of a gel showing all fragment samples used in this section. Section 3: You examine another individual with Dra Ⅰ revealing the following fragment results:
9.0, 5.0, 3.5. You think that this is a bit odd. So, you conduct a few more digests, getting the
following results: Apa Ⅰ 11.5, 4.0, 2.0 Cla Ⅰ 11.3, 6.2 Where is this phenomenon affecting the mtDNA molecule? Indicate the portion of the
molecule that is affected. What fragments would you expect to result from a Cla Ⅰ/Dra Ⅰ
double digest on this individual?
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