how can you test to see if an enzyme was completely saturated

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how can you test to see if an enzyme was completely saturated during an experiment

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Explanation & Answer

Increase the substrate concentration some more. If the rate of the reaction increases, the enzyme was not completely saturated. If the rate of the reaction doesn't change, then the enzyme was already working at V max, and so was already saturated.


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I was struggling with this subject, and this helped me a ton!

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