Hi, I'm looking for help in adding the following features to my program:
1- My problem is I'm not getting the accurate result for the D value it should be D= 244.8My problem is I'm getting D= 229.3 whereas it should be D= 244.8 and eveytime I change the fitting equation I get different value.(please see the attached fitting equation) when I used 0.1 to represent the (r/z)2 I got D= 229.3 whereas using (1/3)**2 gave me D= 298.so I hope you could figure out what's wrong I'm doing and how do I have to write this equation to get D= 244.8 or if you catch another error within my program ,please let me know.
Also , I need the following features to be added:
background signal and make it flexible to change its value from the GUI.
particular file I will send I need the D value to be
D= 244.806 with
background signal = 0.000078 .
corrMath.py file I have FCSCorLen=100 what I need to do is make it flexible to
skip some points like, skipping the first point by entering 1 in the box
assigned for this task in the GUI.
Now, I'm attaching three files (corrMath.py, fitting.py and guiFront.py) please save them in one file and run the guiFront.py you will get the GUI window hit open and choose the file that you can download from this link : https://www.dropbox.com/s/peakwlhwfasl2tn/sample3%20100nM%20543%2020%20per%2020uspixel%20244.806_C001.tif?dl=0
and then hit calculate and then fit and you will see the D value that I'm talking about.