- Home >
- Programming >
- python programming and signal processing
python programming and signal processing
Question Description
Hi, I'm looking for help in adding the following features to my program:
1- My problem is I'm not getting the accurate result for the D value it should be D= 244.8My problem is I'm getting D= 229.3 whereas it should be D= 244.8 and eveytime I change the fitting equation I get different value.(please see the attached fitting equation) when I used 0.1 to represent the (r/z)2 I got D= 229.3 whereas using (1/3)**2 gave me D= 298.so I hope you could figure out what's wrong I'm doing and how do I have to write this equation to get D= 244.8 or if you catch another error within my program ,please let me know.
Also , I need the following features to be added:
1. Adding a background signal and make it flexible to change its value from the GUI.
2. For the particular file I will send I need the D value to be
D= 244.806 with background signal = 0.000078 .
3. In my corrMath.py file I have FCSCorLen=100 what I need to do is make it flexible to skip some points like, skipping the first point by entering 1 in the box assigned for this task in the GUI.
Now, I'm attaching three files (corrMath.py, fitting.py and guiFront.py) please save them in one file and run the guiFront.py you will get the GUI window hit open and choose the file that you can download from this link : https://www.dropbox.com/s/peakwlhwfasl2tn/sample3%20100nM%20543%2020%20per%2020uspixel%20244.806_C001.tif?dl=0
and then hit calculate and then fit and you will see the D value that I'm talking about.
https://www.dropbox.com/sh/ao4cofbu78ey9ku/AACI6Mn8lEIGrBlSJfHT8yKOa?dl=0
This question has not been answered.
Create a free account to get help with this and any other question!
Similar Content
Herzog
by Saul Bellow
12 Rules for Life
by Jordan Peterson
Hidden Figures
by Margot Lee Shetterly
The Odyssey
by Homer
Crime and Punishment
by Fyodor Dostoyevsky
I Cant Make This Up - Life Lessons
by Kevin Hart
A Separate Peace
by John Knowles
The Knife of Never Letting Go
by Patrick Ness
The Picture of Dorian Gray
by Oscar Wilde
Studypool values your privacy. Only questions posted as Public are visible on our website.
Brown University
1271 Tutors
California Institute of Technology
2131 Tutors
Carnegie Mellon University
982 Tutors
Columbia University
1256 Tutors
Dartmouth University
2113 Tutors
Emory University
2279 Tutors
Harvard University
599 Tutors
Massachusetts Institute of Technology
2319 Tutors
New York University
1645 Tutors
Notre Dam University
1911 Tutors
Oklahoma University
2122 Tutors
Pennsylvania State University
932 Tutors
Princeton University
1211 Tutors
Stanford University
983 Tutors
University of California
1282 Tutors
Oxford University
123 Tutors
Yale University
2325 Tutors