Biology................

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timer Asked: Jul 6th, 2015

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PA_Biology_Sugar_Solubility_directions_FINALC.docx <-DIRECTIONS


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Biology Lab Sugar Solubility Lab Lab Instructions In this lesson, you have learned how enzymes work as catalysts in chemical reactions. Enzymes lower the activation energy needed for a chemical reaction to progress, often speeding up a reaction. Throughout the unit, you learned about water’s remarkable property as a universal solvent. Today, we are going to put these two ideas together. As you know, water is not an enzyme. Can we change water to enable it to dissolve a substance faster, the same way an enzyme allows a chemical reaction to go faster? Today, you are going to conduct a lab that demonstrates sugar solubility in water at different temperatures. Please make sure you read over the entire set of instructions BEFORE starting this laboratory investigation. As with any lab, please remember SAFETY FIRST. Start with a clean place to work, follow the directions explicitly, and ask for an adult’s help if you need it. Also, remember that you may email your teacher at any time for help or if you have questions. This lab will be comprised of two parts: 1. Performing the actual sugar solubility lab, which will include conducting the experiment and collecting data 2. Writing your Formal Lab Report with your results and conclusions a. The Formal Lab Report will be the only document you will upload for this lab. You will type directly into this Microsoft Word document. Handwritten documents, PDFs, and image files will NOT be accepted. Multiple files will NOT be accepted. b. Before you start the lab, make sure that you have downloaded the documents listed below from Unit 1 Lesson 10 and saved them to your computer. i. Sugar Solubility Lab Report (Make sure you include your name in the file name. For example: Sugar_Solubility_Lab_Report_John_Doe) ii. Sugar Solubility Lab Results 1. You may also wish to print a copy of each document to 2. write on when you are conducting your lab (especially your Lab Results spreadsheet). However, you may not submit the handwritten document for credit. You must type any notes you take on that copy into the Formal Lab Report. Lab Instructions: Follow the procedures for the lab below. Gather the following materials: • • • • • • • • • • Three clear drinking glasses (You will need to clean and rinse in between temperature trials.) drink pitcher or large pot (large enough to hold 10 cups of water, or a little more than a ½ gallon) liquid measuring cup (1 cup) measuring spoon (1 teaspoon) sugar spoon for stirring butter knife for leveling teaspoon water (You will be adjusting the temperature of water so that you have each of the following. However, do not bring the water to each temperature until right before you will use it. The procedure will explain how to create these temperatures.) o ice water o cold tap water o room temperature water o hot tap water Lab Results sheet downloaded from the lesson (Sugar Solubility Lab Results) Formal Lab Report template from the lesson (Sugar Solubility Lab Report) Always check with an adult before you begin. Preparation Procedure: • Two hours before you start this lab, set at least five cups of water out to reach room temperature. • Approximately 5 minutes before you start the lab, in your drink pitcher or large pot, dump about 4 cups of ice into 6 cups of cold water and stir. Set this aside to use later. • While you are waiting for the ice water to reach the correct temperature, form a hypothesis by predicting which water temperature will dissolve the most sugar and which water temperature will dissolve the least sugar. a. Record this hypothesis on your Lab Results spreadsheet. You may want to explain why you came up with this prediction. Ice Water Procedure: 1. Start with the ice water. Measure one cup of ice water (without ice in it) into a clear, clean drinking glass. 2. Using the measuring spoon, measure out one teaspoon by scooping a heaping amount of sugar into the spoon. a. Scrape the butter knife across the spoon to level out the sugar. (You may have seen someone in your family do this with flour when baking.) 3. Dump the teaspoon of sugar into the water while you stir the water with the spoon. a. It would be great if you had a lab assistant for this. This would be a great way to get your family involved! b. This starts your count. 4. Stir for ten seconds and then continue adding teaspoons of sugar to the ice water until you notice sugar grains starting to collect at the bottom of the glass. a. Be sure to count each teaspoon used. i. You may want to use hash marks on your paper so you do not lose count. ii. Example b. Once you reach this point, stir the water for another 10 seconds to make sure you have reached the saturation point (the point in which the water will not ‘hold’ any more sugar in solution). 5. Record your results in the spreadsheet for trial 1 under “Ice Water.” 6. Repeat the entire procedure two more times for a total of three trials for the ice water. a. Record each trial in your spreadsheet. Cold Tap Water Procedure: 1. Turn on the cold water at your sink and let it run for a few seconds. 2. Measure out one cup of this cold tap water and dump it into a clear, clean glass. a. Make sure the glass is neither heated nor chilled. 3. Repeat steps 2 through 6 from the “Ice Water Procedure” above, recording your results under “Cold Tap Water” in your spreadsheet. Room Temperature Water Procedure: 1. Gather the water you set out a few hours ago. 2. Measure out one cup of this room temperature water and dump it into a clear, clean glass. a. Make sure the glass is neither heated nor chilled. 3. Repeat steps 2 through 6 from the “Ice Water Procedure” above, recording your results under “Room Temp. Water” in your spreadsheet. Hot Tap Water Procedure: Be careful! SAFETY FIRST! 1. Turn on the hot water at your sink and let it run for a few seconds. 2. Measure out one cup of this hot tap water and dump it into a clear, clean glass. a. Make sure the glass is neither heated nor chilled. 3. Repeat steps 2 through 6 from the “Ice Water Procedure” above, recording your results under “Hot Tap Water” in your spreadsheet. ➢ When the spreadsheet is complete, follow the instructions on the Lab Results sheet to produce a “Column Graph” to be used in your Formal Lab Report. o If you prefer, you can make the graph on your own using the features in your word processing program or a drawing program. Just be sure it is accurate and that the graph can be imported into your Formal Lab Report. (It must be inserted into the Formal Lab Report document and not uploaded as a separate document.) ➢ Analyze your results and state your findings by answering the conclusion questions in your Formal Lab Report. ➢ When your lab report is complete, be sure to save it and upload it for credit. Enjoy! . Purpose: In this section, write a brief paragraph stating the purpose and objective of this experiment. What is the purpose of your experiment? What question are you trying to answer? Why are you doing it? What is the information you want to know? Hypothesis: In this section, you will make your hypothesis statement. You need to make an educated guess about the results of your experiment. o Which water temperature do you think will dissolve the most sugar? o Which water temperature do you think will dissolve the least sugar? o Why have you made these hypotheses? Materials: In this section, list all the materials you used throughout your experiment. Data Results: In this section, insert your spreadsheet and the column graph. Conclusion: In this section, you will come to some conclusions about what your data observations and results represent. Analyze your spreadsheet and column graph to answer the following questions. 1) Which water temperature dissolved the most sugar? a. Why do you think this is? 2) Which water temperature dissolved the least amount of sugar? a. Why do you think this is? 3) Did your results support your hypotheses? 4) Why did you have to make sure the glasses were clean and neither heated nor chilled? 5) What was the solute and solvent in this experiment? 6) Do a little research and explain what happened at the molecular level? (You must cite your sources for credit.) 7) Why do you think it was necessary to run three trials at each temperature?
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