I need help with chemistry

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TCOTVEY13

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I need you to make a 6 graphs of my data using excel and post them here. The two pictures will be of the data I have. The the third picture will be instructions of how to use to data. They last picture will be my assignment I have to fill out. 


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11 EXP NUMBER EXPERIMENT/SUBJECT DATE NAME Spectrometrie determination of COURSE & SECTION NO. 020 LAB PARTNER LOCKERIDESK NO. Claire colgrove procedure beverage whose absorbances overlap or dont overlap 1) select beverage, record name, flavor, serving Sho, volume Conc (GlmL Abs (A LOOSAU 0.145 AU 0.30SAU 23 0.482 AU a.632 AN 0,804 AU 10.23ZAU FO.JOSAU al water multi component system 710511S ove oschen Schmidt & Angeinna Lab Purpose: To Quentify food ducs Data/observations Beverage: Gatorade Flavor Orange Serving Size: 1 bottle Volume: 591ML 2) using conc stock of dye H I Dyes: Yellow S, Red 40 prepare standard solutions in dye H I yellow 5 max graduated cylinder Volume added 0.000 (0:1) 424.Snm 3) repeat for dye #2 2 mL sul 424.5 nm 4me sol 424.5 nm 4) Prepare spectrometer as usual (amax) GML Soi 424.5mm 8mL sol 4245 nm SOT 424.5 nm 5) Record Abs for std curve points Tome 14245m (obtain all dye HI 6 points) beverage 0.424,50m 6 obtain absorbonce rave for dye #2 Red 40 volume added End (GMC) a max Abi (AU) beverage 9.000 COD) 2 ml Solu 7) Repeat for dye #2 4 ml sol Solso nm 6ml sol sol.so nm 0.438 AU Experiment H2 8m sol Solso nm 0.57 SAU lomi sol sol.50 nm 0.818 AU 1) Std dilutions are already made I beverage sol.sonm 0.124 AV 2) obtain a max for yellows s red 3 using its PPM 3) Record Abs vawes for all y6 slds (4 data points) and at y6 l max 1o0SAU sol-soam sol.se an 0. 14 2 PU 10.300 AU 니 4) Record Abs values for all Y6 stas (u dota pis) at R3 lmax sauny muy uume 711415 DATE WITNESS/TA NOTE: INSERT DIVIDER UNDER COPY SHEET BEFORE WRITING THE HAYDEN-MCNEIL STUDENT LAB NOTEBOOK Imax conc (g/mL Abs (Au) -0.0SAU 0.145 AU 0.30SAU 3 Beverage: Gator Flavor Orange Serving Size: 1 bottle volume: 591ML Dyes - Yellow 5, Red 40 dye # 1 yellow 5 Volume added 0.000 (17) 424.Sam 2 mL sul 424.5 nm 4 me sol 424.5 m GML Sol 424.5nm 8m. Sol 424.5 nm S Tom 1424.5 nm beverage 14245 AM 0.484.5mm dye #2 Red 40 volume added Q-000 CAD End (gh() & max Abi (AU) 2 ml solu sol-soam solsem 0.142 AU 4ml sol 10.300 AU GML sol 10.438 AU 8ml sol Solso nm 0.57 SAU sol.so nm loml Sol Je 0.818 AU beverage sol.sonm 0.124 AV 0.482 AU 0.632 AN 0,804 AU SO 10.23ZAU 10.00 SAU a wotex =o0SAU Solso nm sol.so nm 0.25W 0,170 PW Yellow Conc avs 96 v) 15ppm 0,626 AU loppm ) 0.434 NU 5ppm 0,226 idi ny nown 0,29960 mas 4%0.56 ///////// //////// //// 3 - Araz Ped 3 conc ars a 16 abs ar3 ispon 0,32SAU lopen 0.346 AU 0, 198 AU 0.140RU 0.6391 sppon 0,0TAU DI To 11.006 AU unknown llllllllll/10420 AU amay II I 529.52m 6) unknown IAZ Analysis Prepare and print the following graphs using Excel or the LabQuest unit, as your instructor 1 directs Beverage graphs (identity & quantify the food dye in your beverage) Graph A Absorbance at the max for the food dye in your beverage versus dye concentration Graph B: Absorbance at the Amax for the 2nd food dye in your beverage (if any) versus concentration Yellow 6 / Red 3 graphs (quantify the dyes with & without considering "interference"): Graph 1: Absorbance at the max for Yellow 6 vs Yellow 6 concentration Graph 2: Absorbance at the max for Red 3 vs Yellow 6 concentration Graph 3: Absorbance at the max for Yellow 6 vs Red 3 concentration Graph 4: Absorbance at the max for Red 3 vs Red 3 concentration Note that Absorbance is conventionally on the (y-axis) and concentration on the (x-axis). 2. Include your measurement for water as a data point in all graphs. Record the best-fit line for each graph; there should be a linear relationship between absorbance and concentration, as dictated by Beer's Law Rearrange Beer's Law (A = &c, where b=1) to E = A/C (4) and note that A/c = Aylax on your graph, thus [ε = slope. As the absorbance of a given species has a different & at each wavelength of interest, each of your plots yields you a specific value for ε for that dye at one particular wavelength. Label your graphs well so that the species and wavelength are clear (informative title & axes labeling). 3. You should confirm that the absorption from one food dye in your beverage does not overlap appreciably with the absorption from another, thus the concentration of each dye in the beverage solution may simply be calculated in a similar fashion. Take care to select ε correctly (i.e., from the appropriate standard curve). Determine the % dye by mass and the mass of dye per serving. 4. Next, calculate the concentration of FD&C Yellow 6 and Red 3 in your unknown mixture using Equation 4, as well. Be careful to select the correct ε to use for your calculations. Performing this “simple” calculation assumes that the absorption from FD&C Yellow 6 does not overlap appreciably with the absorption from Red 3. 5. In reality, as you'll see when you look at your data from this experiment (Part III), the absorption spectra for FD&C Yellow 6 and Red 3 do indeed overlap to some extent. The most accurate analysis of the data, then, will take this into account. In equation form, this may be expressed as: (5) Aunk = AY6 + ARed3 To account for this interference, solve the following simultaneous equations for the unknown concentrations of Yellow 6 and Red 3 (Cy6 and Cred3 respectively). (6a) EY6@amax(Y6)CY6 + ERed3@amax(Y6) Cred3 Aunk@amax(Y6) (6b) = Ey6@imax(Red3) Cve + Ered3@ảmax/Red3,Cred3 Aunk@max(Red3) 63 POSTLAB DISCUSSION - MULTI-COMPONENT SYSTEMS This form should be filled out using PEN only. Mistakes should be crossed through with a single line only Note: all calculations must be shown in lab notebook pages to receive credit TABLE Y DYES FOUND IN Gatorade (input name of selected beverage) Dye Color max (nm) z atamax For % Dye By Mass of Dye Dye Color Mass Per Serving yellows 424.50 ned 40 sol.so TABLE 2: YELLOW 6 & RED 3 DYES Dye Color amax (nm) e at Imax For Concentration Concentration & atumax For Yellow 6 Red 3 (ppm), No (ppm), With Interference* Interference * Yellow 6 480.so Red 3 529.52 *This calculation assumes there is no spectral interference (overlap) between Yellow 6 and Red 3; concentration is calculated simply using the equation of the line at each respective Imax- **This calculation accounts for the spectral interference that does exist between Yellow 6 and Red 3; concentration is calculated using a system of two equations to solve for two unknowns. 1. Which of the following statements are TRUE about the extinction coefficient, €? (Check all that apply.) a. b. C. The extinction coefficient is a measure of the absorbance of a 1.0 M solution of a given species. The extinction coefficient can be obtained from the slope of a Beer's Law plot. The extinction coefficient increases as path length increases. The extinction coefficient is both wavelength and species dependent. For a given chemical species, the extinction coefficient changes with the wavelength of light. d. e. 2. A student is preparing a calibration curve for the absorbance of a colored sports drink. However, the student does not realize that the color of the sports drink is actually composed of two different species of food dye, both of which absorb at the wavelength chosen for the curve. What will happen to the student's calibration curve? a. The data will be skewed high; the calibration curve has positive error. b. The data will be skewed low; the calibration curve has negative error. c. There will be no effect on the data; the calibration curve remains valid. d. The data will be skewed high but, since the error is consistent for all points, the calibration curve remains valid Analysis Prepare and print the following graphs using Excel or the LabQuest unit, as your instructor 1 directs Beverage graphs (identity & quantify the food dye in your beverage) Graph A Absorbance at the max for the food dye in your beverage versus dye concentration Graph B: Absorbance at the Amax for the 2nd food dye in your beverage (if any) versus concentration Yellow 6 / Red 3 graphs (quantify the dyes with & without considering "interference"): Graph 1: Absorbance at the max for Yellow 6 vs Yellow 6 concentration Graph 2: Absorbance at the max for Red 3 vs Yellow 6 concentration Graph 3: Absorbance at the max for Yellow 6 vs Red 3 concentration Graph 4: Absorbance at the max for Red 3 vs Red 3 concentration Note that Absorbance is conventionally on the (y-axis) and concentration on the (x-axis). 2. Include your measurement for water as a data point in all graphs. Record the best-fit line for each graph; there should be a linear relationship between absorbance and concentration, as dictated by Beer's Law Rearrange Beer's Law (A = &c, where b=1) to E = A/C (4) and note that A/c = Aylax on your graph, thus [ε = slope. As the absorbance of a given species has a different & at each wavelength of interest, each of your plots yields you a specific value for ε for that dye at one particular wavelength. Label your graphs well so that the species and wavelength are clear (informative title & axes labeling). 3. You should confirm that the absorption from one food dye in your beverage does not overlap appreciably with the absorption from another, thus the concentration of each dye in the beverage solution may simply be calculated in a similar fashion. Take care to select ε correctly (i.e., from the appropriate standard curve). Determine the % dye by mass and the mass of dye per serving. 4. Next, calculate the concentration of FD&C Yellow 6 and Red 3 in your unknown mixture using Equation 4, as well. Be careful to select the correct ε to use for your calculations. Performing this “simple” calculation assumes that the absorption from FD&C Yellow 6 does not overlap appreciably with the absorption from Red 3. 5. In reality, as you'll see when you look at your data from this experiment (Part III), the absorption spectra for FD&C Yellow 6 and Red 3 do indeed overlap to some extent. The most accurate analysis of the data, then, will take this into account. In equation form, this may be expressed as: (5) Aunk = AY6 + ARed3 To account for this interference, solve the following simultaneous equations for the unknown concentrations of Yellow 6 and Red 3 (Cy6 and Cred3 respectively). (6a) EY6@amax(Y6)CY6 + ERed3@amax(Y6) Cred3 Aunk@amax(Y6) (6b) = Ey6@imax(Red3) Cve + Ered3@ảmax/Red3,Cred3 Aunk@max(Red3) 63
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