Biology experiment outline

Anonymous
timer Asked: Mar 25th, 2017

Question Description

Please review the attachment carefully. The very first paragraph I need a description to post for my discussion thread. The yellow highlighted is the outline for the final assignment and the red highlighted is the final assignment. Also pay close attention to the Material I have to perform the lab assignment. The only part I need soon is the outline part in yellow and the discussion thread. When the final assignment is due I will re-post the assignment so you will be able to win the second part of the assignment. AGAIN PLEASE READ AND PAY CLOSE ATTENTION TO THE INSTRUCTIONS!!!

Topic 5 Getting Ready for the Final Applied Lab Project As you prepare the outline for the final applied lab project this week share with the other students what type of experiment you plan to do. Do not post your entire outline, just include a brief description of the type of enzyme you plan to use, how you will measure enzyme activity and what type of treatments you will use. You may also post your hypothesis to get feedback. Outline Final Applied Lab Project Instructions Include the following in your outline: • • • • • • • • • Name of enzyme you will use Name of organism (if applicable) The substrate and products in the chemical reaction Method for measuring enzyme activity Treatment: acidic fluid(s), pH, length of exposure, how you will treat your samples The control(s) in the experiment Hypothesis How you will present your data (table and/or type of graph) Anything else you would like to get feedback on before you start your experiment Outline includes all the required components and planned experiment meets requirements for the assignment and everything is clearly and accurately described. Final Project Addresses course outcomes 1-4: • • • • recognize and explain how the scientific method is used to solve problems make observations and discriminate between scientific and pseudoscientific explanations weigh evidence and make decisions based on strengths and limitations of scientific knowledge and the scientific method use knowledge of biological principles, the scientific method, and appropriate technologies to ask relevant questions, develop hypotheses, design and conduct experiments, interpret results, and draw conclusions This is the culminating lab assessment in BIOL 103. It is designed to assess your ability to apply the principles of the scientific method. The Effect of low pH on Enzyme Activity Design an experiment in which you will test the effect of an acidic fluid on enzymatic activity. Recall: enzymes are proteins! To complete this project, it may be useful for you to first review the Scientific Method Tutorial, information about pH and enzymes in the text book and course modules, Lab 1 (Introduction to Science) and Lab 4 (Enzymes). As you review Lab 4, you will be reminded that there are several factors that impact enzymatic activity: pH, temperature, and amount of reagent. It is OK to use the same enzyme/substrate/method as you did in lab 4 (but modify the treatment), or you can search on-line to find a different enzyme/substrate/method for measuring enzyme activity for your project (include all references). As you design your experiment for this project, please remember that you are trying to examine how an acidic fluid will modify the outcome of an enzymatic reaction. To successfully complete this project, you will need to identify the question(s) being asked in your experiment and the hypothesis that you are testing. In your experimental design, you must clearly explain what you are doing. That means that you will need to identify the enzyme, the substrate, the acidic fluid used as treatment, the control treatment and the method of measuring enzyme activity, as well as explain your experimental protocol. You must also thoroughly explain how the acidic fluid impacted enzyme activity based on the results from your own experiment as well as knowledge of enzymes and pH from the text book, modules, lab manual and potentially additional information sources. Hint: Keep in mind that the acid will change the environmental conditions of the experiment (for example, a low pH value could change the shape of the active site on the enzyme protein), without directly participating in the reaction. Lab Materials You may need all or some of the following, depending on your experimental design: Materials from your lab kit: • • • • • • • • pH paper hydrogen peroxide solution (you can purchase this at a pharmacy if you have used up the bottle that came with the lab kit) plastic beakers or cups vinegar yeast (can be purchased at grocery store if more is needed) balloons plastic bottle marker for labeling of beakers Write a lab report that includes the following: 1. Title page: descriptive title, your name, course name, semester 2. Introduction: general background information about enzymes and specific information about your chosen enzyme, the question(s) that you are asking and a clear hypothesis for your experiment (20 points). 3. Design an experiment. Provide a detailed description of the materials and methods used to conduct the experiment. Identify control and experimental samples, as well as independent and dependent variables. Also include the methods used for data collection and analysis (20 points). 4. Conduct the experiment and record your results. Take picture(s) of your results. What did you observe? Present your data in table and/or graph format. Remember to label everything and include the unit of measure with all numbers (20 points) 5. Use your knowledge of enzymes and pH to interpret and discuss your results. It may be necessary for you to refer to the OLI course modules, lab manual and/or use additional information resources. What effect does the acidic treatment have on enzyme activity? Did you get the expected results? Explain. (20 points) 6. State a specific and accurate conclusion. Is your hypothesis supported by the results? Looking back, how could you have improved your experiment? (10 points) 7. Include a list of references to all information sources used in APA format (5 points).

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