Interpret these biology experiment results (check attached file):

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timer Asked: Apr 4th, 2017

Question Description

Please interpret these biology lab experiment results:

The hypotheses tested were:

(1) Sampling non-soil environments within the local marsh will result in the isolation of antibiotic-producing organisms that are unusual, relatively unstudied, and/or produce novel antibiotics.

(2) Bacteria in the genus Streptomyces (by far the most diverse producers of bioactive compounds) from non-soil environments are distinct from Streptomyces from soil environments.

Soil/plant/sediment samples were isolated for bacteria, tested against clinically relevant microorganisms, and those who showed inhibitory activity were characterized.

The results are in the file attached.

Unformatted Attachment Preview

Table 1. Inhibition of tester strains by antibiotic producers isolated. An 'X' indicates that tester strain was inhibited. Abbreviations: Acinetobacter baumanii (Ab); Enterobacter aerogenes (Ea); Escherichia coli (Ec); Enterococcus raffinosus (Er); Pseudomonas putida (Pp); Staphylococcus epidermidis (Se). Ab Ea Ec Er Pp Se IX X X X X Name 1A1 1A2 1B1 1B2 ici 1C2 1D1 1D2 1E1 1E2 X X X X X XN X X X X X X X X X X X X X Table 2. Taxonomy based on 16S rRNA genes, and characterization of antibiotics produced by isolates. 1000-nucleotide long alignments were produced using BLASTn, and % of identical nucleotides with closest BLAST match in Genbank are depicted. Various methods (see supplement) were used to characterize the chemicals produced by the isolates. Name 1A1 1A2 1B1 1B2 1C1 1C2 1D1 1D2 1E1 1E2 16S rRNA ID (% identity) Streptomyces platensis (99%) Micromonospora purpurea (98%) Pseudomonas fluorescens (99%) Streptomyces fradiaea (98%) Streptomyces aureofaciens (98%) Pseudomonas fluorescens (99%) Streptomyces griseus (99%) Actinomycetes sp (87%) Pseudomonas fluorescens (99%) Streptomyces griseus (99%) Chemicals Detected Platensimycin Gentamicin Mupirocin Fosfomycin Tetracycline Pyoluteorin Streptomycin Multiple unknown 2,4-diacetylphloroglucinol kanamycin
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