Lab 1 – Introduction to Science Exercise 1: The Scientific Method In this exercise, you will answer the questions based on what you have seen in the videos throughout the lab. Be sure to pay careful attention to the videos – you will not only need them to complete this exercise successfully, but also to have a firm understanding of the scientific method for future labs. QUESTIONS 1. Make an observation – Write down any observations you have made regarding the effect of pollution on the environment. Answer = Led in Flint Michigan. Auto Exhaust on air quality. Oil Spills in the Gulf Coast. Pollution harming the plants that we are growing. Effects the organisms that live in the forests. 2. Do background research – Utilizing the scholarly source (provided here), describe how pollution might affect yeast. Answer = There was an impact on the composition of the Yeast. The pigmented urediniomycetous yeasts in the soil have increased. It seemed like the pollution had prevented the growth to be as good as it has been before. The different Cu levels did take into effect. 3. Construct a hypothesis – Based on your research from question 2, develop an if-then hypothesis relating to the effect of pollution on yeast respiration. Answer = 4. Test with an experiment – Identify the dependent variable, independent variable, and the controlled variables for the experiment. Answer = © eScience Labs, 2016 5. Analyze results – Record your observations of the three test tubes before incubation and compare them to the observations provided in the video. Answer = Test Tube Yeast with No Pollutant Yeast with Salt Water Yeast with Detergent Initial Appearance 6. Analyze results – Record your observations of the three test tubes after incubation. Answer = Test Tube Yeast with No Pollutant Yeast with Salt Water Yeast with Detergent Final Appearance 7. Analyze results – The table below shows sample data regarding the amount of carbon dioxide produced by each tube. Determine what type of graph would be the most appropriate for displaying the data and explain why you chose that graph. Then, make a graph. Use Microsoft Excel or a free graphing program (for example, https://nces.ed.gov/nceskids/createagraph/) to create the graph. Submit this with your post-lab questions. Sample Yeast with No Pollutant Yeast with Salt Water Yeast with Detergent Amount CO2 Produced (mL) After 1 Hour 7 mL 0.5 mL 0 mL Answer = 8. Draw conclusions – Interpret the data from the graph in Question 7. What conclusions can you make based on this graph? Answer = © eScience Labs, 2016 9. Draw conclusions – Based on your observations and your graph, would you reject or accept the hypothesis you made in Question 3? Why? Answer = 10. Draw conclusions – Imagine you are an environmental scientist employed by a city. Some residents have expressed concerns regarding how salt is applied to roadways in the winter because of the harm it may cause aquatic life in area streams. Propose an experiment using yeast to determine if salt pollution runoff is a potential concern in your community. Answer = References Any sources utilized should be listed here. © eScience Labs, 2016
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