Blood lab report for microbiology

Anonymous
timer Asked: Apr 29th, 2017

Question description

I am currently taking an course online for microbiology and we complete labs online using stimulations using Mc Graw hill education website. I have completed the labs and needed help with my labs i have attach the images below pertaining to the lab courses online.

Wymeshia Robinson SCORE Blood Smear and Differential White Cell Count Pre-lab briefing Recall the steps to perform a blood smear 79% 100% 100% Why do you need to hold the slide in a 45 degree angle? Did you know the answer: You said 'Unsure'. You answered correctly: To ensure the proper thickness of the smear Recall how to perform a differential white blood cell count 100% How is the differential white blood cell count determined? Did you know the answer: You said 'Unsure'. You answered correctly: By counting a set number of white blood cells and noting the percentages of different types of cells Identify different white blood cells Identify platelets in a blood smear slide 100% 100% You placed the label "Platelets" correctly in the label game Identify erythrocytes in a blood smear slide 100% You placed the label "Erythrocytes" correctly in the label game Identify neutrophils in a blood smear slide 100% You placed the label "Neutrophil" correctly in the label game Identify lymphocytes in a blood smear slide 100% You placed the label "Lymphocyte" correctly in the label game Identify monocytes in a blood smear slide 100% You placed the label "Monocyte" correctly in the label game Identify eosinophils in a blood smear slide 100% You placed the label "Eosinophil" correctly in the label game Identify basophils in a blood smear slide 100% You placed the label "Basophil" correctly in the label game Simulation of Blood Smear and Differential White Cell Count Prepare a blood smear Add a drop of blood to a microscope slide 67% 100% 100% The point is to add the drop not in the center but at the side of the slide. Use another slide to smear the blood drop 100% This technique allows to spread a drop of blood thinly and evenly across a microscopic sllde. Let blood smear dry in the air 100% Successful preparation means following the steps. Well done! Stain the blood smear Add Wright's stain to blood smear 91% 100% Thi will enable us to clealy identify WBCs later by staining their nuclei. Let Wright's stain react with blood smear for 2 minutes Add distilled water to the slide with stain 50% 100% This ensures that excess stain is removed and we do not have to deal with stain artifacts when we view the slide under the microscope. Let the stain and water mixture react with the stain for 4 minutes 100% This ensures that excess stain is removed and we do not have to deal with stain artifacts when we view the slide under the microscope. Rinse the stained blood smear 100% This ensures that excess stain is removed and we do not have to deal with stain artifacts when we view the slide under the microscope. Let the slide air dry for at least one minute 100% A dry, well stained slide will provide the best viewing results. Perform the correct procedure without guidance 100% In a real lab environment, attention to detail and repeating the same procedure many times guarantees helps to follow all the steps without any guidance. Dispose of materials contaminated with blood in biohazard container 100% This is not only the safety requirement by law but it makes sense in the light of widespread presence of infectious bloodbourne diseases. Perform a differential count on prepared microscope slide 100% This tells us whether the different types of WBCs are in the expected proportions or not. If not, it provides an important clue as to what is going on in the patient's body. Differential cell count 0% You haven't mastered this yet. Count the correct number of neutrophils 0% Having a clear idea of what a neutrophil looks like and not going over the same area more than once are the two essentials for doing a correct count. Remember that neutrophils are "polymorphonuclear" and granulocytes. Count the correct number of lymphocytes 0% Having a clear idea of what a lymphocyte looks like and not going over the same area more than once are the two essentials for doing a correct count. Remember that lymphocytes have a large, round nucleus and are agranulocytes. Count the correct number of monocytes 0% Having a clear idea of what a monocyte looks like and not going over the same area more than once are the two essentials for doing a correct count. Remember that monocytes are larger than RBCs, have a distinct bean shaped nucleus and are agranulocytes. Count the correct number of eosinophils 0% Having a clear idea of what an eosinophil looks like and not going over the same area more than once are the two essentials for doing a correct count. Remember that eosinophils are granulocytes full of reddishpink staining granules and a bilobed nucleus. Count the correct number of basophils 0% Having a clear idea of what a basophil looks like and not going over the same area more than once are the two essentials for doing a correct count. Remember that basophils are granulocytes full of dark purple staining granules with a bilobed nucleus. Infer the patient's health problem from the results of the differential white cell count 100% With the differential count showing the leukocyte types in roughly the normal proportions, this patient is obviously normal regarding their white blood cells. Post-lab probing Explain the outcome if the stain acts for the wrong time 100% 100% We need as much Wright's stain as ____________. Did you know the answer: You said 'Unsure'. You answered correctly: is necessary to cover all the blood smear What will happen if the Wright's stain reacts with the slide for less than a minute? Did you know the answer: You said 'Unsure'. You answered correctly: Weak staining and poor contrast Identify the normal values of a differential white blood cell count 100% Match the percentages to the correct type of leukocyte as we would see it in a healthy individual. Did you know the answer: You said 'Maybe'. You form the correct answer: 50%-70% is matching with Neutrophils 25%-35% is matching with Lymphocytes 4%-6% is matching with Monocytes 1%-3% is matching with Eosinophils 0.4%-1% is matching with Basophils Know the relationship between an abnormal differential white cell count and likely 100% diseases What problem is most likely the case when a person has a high count of eosinophils? Did you know the answer: You said 'Unsure'. You answered correctly: • Parasitic infection • Allergic reaction That typically elevates the eosinophil count
Wymeshia Robinson SCORE Blood Typing Test Pre-lab Briefing Recall how to determine the blood type 82% 100% 100% The picture shows a test result which indicates blood type _________. Did you know the answer: You said 'No idea'.The correct answer is: B+ Recall which transfusions lead to transfusion reactions 100% Which of the following statements best describes what you would expect to happen in a packed cell transfusion if the recipient had blood type A and the donor had blood type 0? Did you know the answer: You said 'Unsure'. You answered correctly: There would be no transfusion reaction Which of the following statements best describes what you would expect to happen if a transfusion recipient had blood type 0 and a donor had blood type AB? Did you know the answer: You said 'Unsure'. You answered correctly: Agglutination would occur as the recipient's antibodies bind to the donors antigens Simulation of Blood Typing Test Test all blood samples 78% 100% All three samples have different results. Label the test slides 100% One of the rules of good lab work is to clearly label all unmarked items. This allows clear evaluation even if a distraction does occur. Recall how the slides should be labeled 0% Add information that ties the slide with the patient the sample was drawn from, and with the tests that will be done to it. Add blood from only one patient to each slide 100% One of the rules of good lab work is to clearly label all unmarked items. This allows clear evaluation even if a distraction does occur. Add sera to the spots labeled for them 100% One of the rules of good lab work is to clearly label all unmarked items. This allows clear evaluation even if a distraction does occur. Determine the blood type 32% Remember to wait for 2 minutes. Then click on the + sign to see a sufficiently enlarged picture. Compare each field on the slide with the respective row on the evaluation chart. Use safe blood handling practices 50% Disposing of blood stained materials in the Biohazard container is standard safety procedure when handling blood Avoid cross-contaminating blood samples 100% Clear labeling and systematic work help to prevent crosscontamination - which is the enemy of reliable results. Recall why toothpicks are used in this experiment 100% Use of separate toothpicks to mix the serum and blood helps to obtain correct results. Use your results to determine who can donate blood to whom 67% The blood typing test is used to determine who a person can donate blood to, and from whom he or she can receive a blood donation. Post-lab Probing 100% Realize the need for type O packed cell transfusion when donor and recipient do not 100% exactly match When donor and recipient have the exact same blood type, like B+ for example, their blood will obviously match. What if the recipient type does not match the donor type? Did you know the answer: You said 'No idea'.The correct answer is: • Any recipient can receive type O red blood cells (packed cells) but not the type O plasma • A Rhesus positive recipient can always receive Rhesus negative blood of the same type. (A+ can receive A- blood for example.) • Recipients with type AB+ can receive any type of donor blood cells (packed cells), but not any type of plasma Correct. The cells contain no antigens so whatever antibodies a recipient may have, they have nothing to attach to. However, Type O blood containsAantiand antiB antibodies and thus there would be a mild clumping reaction with some of the recipient's red blood cells. Correct. Packed cells of any type are ok as the plasma of a type AB person does not contain any antibodies for A or B. However, if the donors blood is not also AB then their plasma contains antibodies which would react with the recipient's red blood cells. Yes. Rhesus negative blood does not contain antibodies against Rhesus factor unless the Rh- donor had been exposed to Rh+ blood earlier in their life.
Wymeshia Robinson SCORE Hemoglobin Content Pre-lab Briefing Recall how to prepare a blood sample for a hemoglobin test 85% 100% 100% When you move the slide in the hemoglobinometer, your goal is to _______ the two colors. Did you know the answer: You said 'Maybe'. You wrote the correct answer: match You need to stir the blood with the hemolysis applicator _________. Did you know the answer: You said 'Unsure'. You answered correctly: until it appears clear, not cloudy Simulation of Hemoglobin Test Test the three blood samples and positive and negative controls 80% 100% Clear benchmarks for normal and pathological as provided by the controls enables accurate analysis of the unknown samples. Stir with hemolysis applicator until all the hemoglobin is out of the red blood cells 100% Only diligent striring will lead to reliable results as otherwise not all hemoglobin was released out of the red blood cells. Measure the hemoglobin content 100% This device works by comparing the probe with a standard and it measures the deviation. You see the result directly in the provided scale. Use safe blood handling practices 0% Disposing of blood stained materials in the Biohazard container is standard safety procedure when handling blood Avoid cross-contaminating samples 0% Always use new utensils for every sample. Blood residue from a former sample will lead to cross-contamination. Recall why hemolysis applicators are used 100% This brute mechanical method works by breaking the RBCs cell membrane. Infer whether test results indicate doping 40% Compare the hemoglobin levels for each sample with the positive and negative test samples. If the hemoglobin level for the positive sample is much closer than for the negative test sample, the athlete is likely to have blood doped. Post-lab Probing Explain the purpose of a hemoglobin test 100% 100% With the hemoglobin measurement we determine one of the important factors that determine _________________ . Did you know the answer: You said 'I know it'. You answered correctly: the oxygen transport capacity of a person's blood Recall the normal hemoglobin content 100% A patient's hemoglobin content is determined to be 10 g/100ml. That is __________________ . Did you know the answer: You said 'Unsure'. You answered correctly: low for both a woman or a man Norm range men: 14-18 g/100ml; women 12-16 g/100ml
Wymeshia Robinson SCORE Hematocrit Pre-lab Briefing Recall how to prepare a blood sample for a hematocrit test 79% 100% 100% Match the number to create the correct sequence of steps in a hematocrit determination. Did you know the answer: You said 'Maybe'. You form the correct answer: 1 is matching with Fill a glass capillary with blood from a disinfected finger tip. 2 is matching with Seal off the capillary on one end with a putty. 3 is matching with Place your capillary into a centrifuge with the sealed end facing outwards. 4 is matching with Balance the centrifuge by loading it evenly. 5 is matching with Spin the capilaries for about 4 min. 6 is matching with Place the capillary over the evaluation grid. Simulation of Hematocrit Test 74% Fill a capillary tube with blood 100% Separate blood and plasma in the centrifuge 100% Seal capillary tubes after adding blood 100% Using a seal and putting the sample into the centrifuge correctly prevents the blood sample from being pushed out of the capillary by the large centrifugal forces occurring during spinning. Measure the hematocrit for one blood sample 100% The evaluation chart makes it easy to instantly read the hematocrit as long as the capillary tube is placed correctly. Test all 5 blood samples 80% In order to evaluate our unknown samples we must first have an idea what a clearly normal and a clealy abnormal sample look like. Those two are our controls. Balance the centrifuge 0% Always balance the load chamber in a centrifuge, as there are strong forces at work during spinning. Recall how to place the capillary tubes in centrifuge 0% Put the end with the seal towards the outer side of the centrifuge. Infer whether test results indicate doping 40% Compare the hematocrit results of the test samples to positive and negative test results. If the test hematocrit results are much closer to the positive hematocrit levels than to the negative levels, the athlete is likely to be blood doped. Clean blood contaminated materials or dispose of them in the biohazard container 100% Good lab safety prevents accidents tha may result in serious infections for lab personal. Avoid cross-contaminating samples 100% Focused work with new utensils for each sample is rewarded by correct results. Post-lab Probing Explain the purpose of a hematocrit test 100% 100% With the hematocrit measurement we determine one of the important factors that determine _______________. Did you know the answer: You said 'Unsure'. You answered correctly: the oxygen transport capacity of a person's blood With the hematocrit test, we determine one of the important factors that determine _______________. Did you know the answer: You said 'Unsure'. You answered correctly: the oxygen transport capacity of a person's blood Recall the normal hematocrit levels 100% A male athlete has a hematocrit of 55% prior to a competition. That indicates ___________________ . Did you know the answer: You said 'No idea'.The correct answer is: an endurance trained male in the norm range Correct: the norm range for average males is between 40-54%. Assuming that that person has been physically very active, a few percentage points above norm are not unexpected. A male athlete has a hematocrit of 55% prior to a competition. That indicates ___________________ . Did you know the answer: You said 'Unsure'. You answered correctly: an endurance trained male in the norm range Correct: the norm range for average males is between 40-54%. Assuming that that person has been physically very active, a few percentage points above norm are not unexpected.

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