Description
Create a practice experiment on an enzyme. Need an hypothesis, and instructions are attached for the practice experiement.
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Explanation & Answer
Attached.
Running head: ENZYME ACTIVITY EFFECT ON LOW PH
Enzyme activity effect on low pH
Student’s Name:
Institutional Affiliation:
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ENZYME ACTIVITY EFFECT ON LOW PH
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Enzyme name
The enzyme for the experiment will be the yeast that is purchased from a nearby store. Its
purpose will be to act as catalase in breaking down the substrate to water and oxygen.
Additionally, they are commonly regarded as protein mainly located in the human body and are
responsible for controlling metabolic reactions.
Substrate
For this investigation Hydrogen peroxide will be the substrate. Since chemicals are impetuses,
they are not altered by the responses they do. In this case the substrate, is changed over to
something. For this situation, hydrogen peroxide is being changed over to water and oxygen.
Hypothesis
Low pH measures result in the reduction of enzyme activity. Exceedingly low pH esteems
accepted to bring about the total loss of activity for general catalysts.
Method
Chemical action will be estimated by evaluating the extent of the inflatables after various
proportions of vinegar to water blended with the substrate, at that point added to yeast (catalyst).
The accompanying is the technique I will use to test the impact of pH levels on enzymatic
movement:
1. Label the beakers from 1 to 3 while placed in a test tube holder
2. Fill each of them with 10 ml of hydrogen peroxide
3. Arrange the balloons in a corresponding manner (use balloons of different colors for each
beaker)
ENZYME ACTIVITY EFFECT ON LOW PH
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4. To each balloon add 0.05 grams of the yeast
5. Add 15ml of 100% vinegar into beaker 1 yielding a pH level of 2 mix and check the pH
level while recording
6. Fit the balloon tightly to beaker 1 ensuring no space for gas escape, once that is done rub
the balloon so that all the yeast goes into the beaker 1with the balloon still attached to the
beaker measure the size of the balloon using a Vinyl tape while recording the data.
7. Add 5ml of 75% vinegar with distilled water into beaker 2 yielding a pH of 4 and mix
well while checking the pH level
8. Fit the balloon tightly to beaker 2 ensuring no space for gas escape, once that is done rub
the balloon so that all the yeast goes into the beaker 2 with the balloon still attached to the
beaker measure the size of the balloon using a Vinyl tape while recording the data.
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