Paper Chromatography Lab

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lhyn2002

Science

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Chemistry Lab with Paper chromatography. No need to insert photos. Not difficult and 1 day to complete

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Paper Chromatography Add your name here Date lab was performed: mm/dd/yy Title Page Purpose of Experiment – 2 Research – 2 Hypothesis –4 Material List –4 Procedure - 2 Data Table & Observations – 4 Graph – 6 Error Analysis – 4 Summary of Results – 6 Conclusion – 6 Total - 40 Fill in all areas highlighted in yellow!!!! Point values for each slide are to the left. This lab has two parts but the lab write-up will be based upon the results in part 2 only. Purpose of Experiment • Testable Question – How does the type of solvent affect the separation of ink mixtures? • Statement with Independent Variable and Dependent Variable – The independent variable in this experiment is the solvent. – The dependent variable in this experiment is the distance the ink has moved up the filter paper strips. Highlight the IV and DV in the statement below. – The purpose of this experiment is to determine which type of solvent will cause the ink mixtures to move through the stationary phase the most. Research • From the Laboratory Guide for Unit 1 Lab • Paper chromatography is used to visibly separate components of homogenous mixtures like ink. A solvent (known as the mobile phase) will travel up the paper strips (known as the stationary phase). The components in the inks will travel up the stationary phase at different rates. Some solvents are more effective at moving the ink mixtures up the stationary phase than others. • You must cite your source (Franck, Daniel. Chemistry: A Laboratory Guide. K12, Inc. Lab 1, Paper Chromatography, pg. 2.) Underline at least four vocabulary words associated with paper chromatograph in the paragraph above. Hypothesis • Your hypothesis is your educated guess. The hypothesis will guide the experiment and must be SPECIFIC. The hypothesis is written as an IF … IV..., THEN … DV…. statement. • There are four different solvents – water, alcohol, water-alcohol mixture, vegetable oil. • Which solvent do you think will work the best at moving the inks up the paper? If _____________ is used as a solvent, then it will move the ink mixtures up the filter paper the greatest distance. Material List and Labeled Diagram • Use material list provided in the lab directions. • Add picture of your lab set-up Procedure Part 2 • • • • • • Cut 12 strips of filter paper 1.2cm x 10cm. Bend all the paper strips so that the bottom of the strip touches the bottom of the microplate wells. Using the black permanent marker, draw a black dot 0.5cm above the paper crease on four of the strips. Repeat for each of the other two marker colors. Use the pipettes to fill the microplate wells with water, water-alcohol mixture, alcohol, & vegetable oil as indicated in the table. Set timer for 20 minutes then record results. Unit 1 Lab - Paper Chromatography Data Table Fill in the Data Table with the distance the INK traveled in centimeters. Black S O Red Green Water L V Water-Alcohol E N T S Alcohol Vegetable Oil Distance INK Traveled (cm) in 20 minutes Unit 1 Lab - Paper Chromatography Graph Use the NCES Create a Graph website - https://nces.ed.gov/nceskids/createagraph/ Choose Bar type and click on the Data tab. Setup and fill in the information as seen below then add your own data to the blank spaces. Click on the Preview tab and take a screenshot of your graph and place below. Place bar graph for part 2 here. Insert picture of results below. • Results of water as a solvent • Results of alcohol as a solvent • Results of water-alcohol as a solvent • Results of vegetable oil as a solvent Error Analysis • • Error analysis is identifying sources of error in your experiment. Error can be identified by looking at your data. Data that seems completely different from other peer results or trials may indicate a source of error. You need to specify which part of the experiment the error was in. • • Write your statement here. Summary of Results Summary of Results consists of three sentences For sentence 1, you have two choices. This has to do with the validity of the experiment. Remember that your hypothesis is an educated guess so you need to state how the hypothesis compares to the outcome of your experiment. You will need to determine which one you will choose based upon your data as it relates to your hypothesis. Choice 1. If your guess was correct. The hypothesis was supported by the outcome of this experiment , therefore the experiment is valid. This means that your hypothesis and outcome were the same. Choice 2. If your guess was incorrect. The hypothesis was not supported by the outcome of this experiment although the question was addressed, therefore the experiment is valid. This means that your hypothesis does not match your outcome but the data shows that the hypothesis was tested appropriately. For sentence 2, you need to restate your purpose. The purpose of the experiment on slide 2 was written before you performed the lab so now you need to change the verb tense to reflect the past tense. Sentence 3 is a statement of what DID happen in the experiment that answers your question on slide 2. Use observations and data to back up your statement. Summary of Results Write your three sentences below: Conclusion The conclusion should include the following: 1. What differences did you observe in how the different colored inks were separated? 2. Compare the effectiveness of each of the four kinds of solvents. 3. Which solvent caused the ink from the dots to move the most? Which solvent caused the ink from the dots to move the least? 4. Use specific evidence (measurements) from the experiment to support your conclusion and vocabulary specific to paper chromatography. 5. Give an example of how paper chromatography is used in the real world. Write your answer in paragraph format.
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