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EGFR paper, basically, you will be given one paragraph, but you will need to write an assay based on the paragraph. After this test, you will be able to read the top sci papers, and know how to write a review.
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cell
by the
the na
and discusses potential therapeutic opportunities and molecu
Fc) emerging targets as well as the technological hurdles of the
his is in raising GPCR-targeting antibodies. We also describe ing to
ment
the growing pipeline of antibody-based drugs that are (ortho
ycia
directed at GPCRs entering clinical development and site (al
gens
pranes
discuss some specific examples.
sterica
nisms
bindir
have
Why target GPCRs with antibodies
lize th
Although GPCRs are the largest class of drug-targeted the ca
molecules, three-quarters of the 'GPCRome' remains teins
undrugged. Only ~80 GPCRs are drugged with small
the an
ector molecules, and close to 30 GPCRs are targeted with active
d by
peptides, usually those related to the natural endo-
ent
genous ligand20. Drug development for this target class agoni
ngly
MD has been beset by difficulties in the identification of tor si
and molecules with suitable selectivity and drug-like prop- disso
CO
erties. Selectivity between related receptors that share a the a
mour
common ligand may be challenging for small molecules, whic
which bind to the conserved ligand-binding site, but may
Thes
be easier for antibodies that are directed at less well-
may
B
cates conserved allosteric sites21. Some of the off-target toxicity
sed of
that has been observed from small-molecule compounds dime
A to
identified from high-throughput screens could be related ger
nd
to high lipophilicity and molecular weight, which is sign
ing
common in drugs that have been derived this way.
lity for
RUG DISCOVERY
© 2017 Macmillan Publishers Limited, part of Springer Nature. Al
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Running head: WHY TARGET GPCRs WITH ANTIBODIES
Why target GPCRs with antibodies
Name
Institution
1
WHY TARGET GPCRs WITH ANTIBODIES
2
The G protein-coupled receptors (GPCRs) still remain a highly targeted class for small
molecule drug production. However, the antibodies are becoming a preferred alternative because
of the GPCRs being intractable to the small molecule drug discovery. Equally, increasing
antibodies to GPCRs has proved a hard task because of the difficulties in getting suitable antigen
given that GPCRs are often unstable and have low levels of cell expressions (Hutchings, Koglin
& Marshall, 2010). The main interes...
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BIBC 102 UCSD Glucose Synthesis Worksheet
1a. Please explain why acetyl CoA cannot be used as a precursor for glucose synthesis in animals (2 points).
1b. Plants an ...
BIBC 102 UCSD Glucose Synthesis Worksheet
1a. Please explain why acetyl CoA cannot be used as a precursor for glucose synthesis in animals (2 points).
1b. Plants and some bacteria, however, can convert acetyl CoA into glucose. Please briefly describe how acetyl CoA is converted into glucose (3 points).
1c. How many NET Acetyl CoA, NET ATP, and NET NADH are needed in order to synthesize ONE molecule of glucose from acetyl CoA in plants (3 points)? Count GTP as ATP if GTP is used or produced and count FADH2 as NADH if FADH2 is used or produced in the process of synthesizing glucose.
1c. Although humans cannot synthesize glucose from acetyl CoA, please explain why research on such pathways in plants and bacteria may have an impact on human health (1 point)
Question # 2 (6 Points total)
An experiment is done to measure electron transport and ATP synthesis in isolated mitochondria. Sketch a plot of the hypothetical experimental results if you measure both O2 consumption and ATP synthesis (Left Y-axis for O2 consumption; Right Y-axis for ATP production, overlay both on the same plot. You may use different color or simply indicate the curves) versus time (X-axis). Start the experiment (t = 0) with succinate, ADP, and Pi in the mix and successively add DNP at t = 4 min., then Oligomycin (at t = 8 min.), and cyanide (at t = 12 minutes). Indicate the points in the plot that each agent is added. (6 points)
Question # 3
Thiols play very important roles in metabolism. List at least four thiol-containing molecules we learned in this course (2 points). Please give at least two examples that a high-energy thioester bond is used to synthesize high energy Pi-containing compounds (2 points)
4. Glycogen metabolism
5a. Please describe how the Red glucose residues and the blue glucose residues are metabolized. (2 points)
5b. When all of the glucose residues marked in red, blue, and green are completely oxidized into CO2 and water, how many NET ATP are produced. Consider 2.5 ATP for each NADH, 1.5 ATP for each FADH2, and count GTP as ATP (4 points)
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Unit II JournalMore states in the United States are legalizing the use of medicinal marijuana. Some states have laws that ...
Columbia Southern University Legalizing Marijuana Research Paper
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HUN 2000 Ras Mussen College Human Nutrition Worksheet
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HUN 2000 Ras Mussen College Human Nutrition Worksheet
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WEAX 201 ERAU Daytona Beach Meteorology Earths Atmosphere Discussion
Choose TWO questions - one from each question set below - to answer. Place your chosen questions and the formulated answer ...
WEAX 201 ERAU Daytona Beach Meteorology Earths Atmosphere Discussion
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BIBC 102 UCSD Glucose Synthesis Worksheet
1a. Please explain why acetyl CoA cannot be used as a precursor for glucose synthesis in animals (2 points).
1b. Plants an ...
BIBC 102 UCSD Glucose Synthesis Worksheet
1a. Please explain why acetyl CoA cannot be used as a precursor for glucose synthesis in animals (2 points).
1b. Plants and some bacteria, however, can convert acetyl CoA into glucose. Please briefly describe how acetyl CoA is converted into glucose (3 points).
1c. How many NET Acetyl CoA, NET ATP, and NET NADH are needed in order to synthesize ONE molecule of glucose from acetyl CoA in plants (3 points)? Count GTP as ATP if GTP is used or produced and count FADH2 as NADH if FADH2 is used or produced in the process of synthesizing glucose.
1c. Although humans cannot synthesize glucose from acetyl CoA, please explain why research on such pathways in plants and bacteria may have an impact on human health (1 point)
Question # 2 (6 Points total)
An experiment is done to measure electron transport and ATP synthesis in isolated mitochondria. Sketch a plot of the hypothetical experimental results if you measure both O2 consumption and ATP synthesis (Left Y-axis for O2 consumption; Right Y-axis for ATP production, overlay both on the same plot. You may use different color or simply indicate the curves) versus time (X-axis). Start the experiment (t = 0) with succinate, ADP, and Pi in the mix and successively add DNP at t = 4 min., then Oligomycin (at t = 8 min.), and cyanide (at t = 12 minutes). Indicate the points in the plot that each agent is added. (6 points)
Question # 3
Thiols play very important roles in metabolism. List at least four thiol-containing molecules we learned in this course (2 points). Please give at least two examples that a high-energy thioester bond is used to synthesize high energy Pi-containing compounds (2 points)
4. Glycogen metabolism
5a. Please describe how the Red glucose residues and the blue glucose residues are metabolized. (2 points)
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VME University of Florida Laboratory of Benzoylecgonine Components Lab Report
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VME University of Florida Laboratory of Benzoylecgonine Components Lab Report
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Columbia Southern University Legalizing Marijuana Research Paper
Unit II JournalMore states in the United States are legalizing the use of medicinal marijuana. Some states have laws that ...
Columbia Southern University Legalizing Marijuana Research Paper
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HUN 2000 Ras Mussen College Human Nutrition Worksheet
For this assignment, use the Nutritional Intake Worksheet (below) to:Familiarize yourself with the 2015-2020 USDA Dietary ...
HUN 2000 Ras Mussen College Human Nutrition Worksheet
For this assignment, use the Nutritional Intake Worksheet (below) to:Familiarize yourself with the 2015-2020 USDA Dietary Guidelines for an adult.Track all of the food and beverages you consume for a 3-day period.Analyze your nutritional intake compared to the USDA Dietary Guidelines.Answer a series of self-evaluation questions.Directions: This worksheet is designed to help you determine how many servings of the 2015-2020 Dietary Guidelines you consume. This will give you a chance to review the recommended amounts, and see how you compare.The 2015-2020 Dietary Guidelines are listed in the chart below and will be used to analyze your food intake.Page 2 contains a food log, where you will record all of the food and beverages you consume.Page 3 contains a table where you will analyze your nutritional intake compared to the USDA Dietary Guidelines and then answer a series of self-evaluation questions. 2015-2020 USDA Dietary Guidelines Note: The following are based on a 2000 calorie diet for an 18+yr old adult according to the 2015-2020 Dietary Guidelines. Dairy: USDA Recommended amount: 3 cups/day What counts as 1 cup: 6 ounce portion of fat-free yogurt is equal to 3/4 cup-equivalent dairy; 1 1/2 ounces portion of cheddar cheese is equal to 1 cup-equivalent dairyWhat counts as 1 ounce:1 large egg is equal to 1 ounce-equivalent protein foods; 2 tablespoons of peanut butter is equal to 2 ounce-equivalents protein foods; 1 ounce portion of walnuts is equal to 2 ounce-equivalents protein food; 1/2 cup portion of black beans is equal to 2 ounce-equivalents protein foods; 4 ounce portion of pork is equal to 4 ounce-equivalents protein foodsWhat counts as 1 cup:1/2 cup portion of strawberries is equal to 1/2 cup-equivalent fruit; 3/4 cup portion of 100% orange juice is equal to 3/4 cup-equivalent fruit; 1/4 cup portion of raisins is equal to 1/2 cup-equivalent fruitWhat counts as 1 cup:1/2 cup portion of green beans is equal to 1/2 cup-equivalent vegetables; 1 cup portion of raw spinach is equal to 1/2 cup-equivalent vegetablesWhat counts as 1 ounce:1 slice of bread is equal to 1 ounce-equivalent grains; 1/2 cup portion of cooked brown rice is equal to 1 ounce-equivalent grainsConsume less than 10 percent of calories per day from added sugarsConsume less than 10 percent of calories per day from saturated fats Protein: USDA Recommended amount: 5.5 ounces/day Fruits: USDA Recommended amount: 2 cups/day Vegetables: USDA Recommended amount: 2.5 cups/day Grains: USDA Recommended amount: 6 ounces/day Sugar: USDA Recommended amount: minimal/day Fat: USDA Recommended amount: minimal/day Retrieved from https://health.gov/dietaryguidelines/2015/resource... on 04/08/2019.Food Log: Nutritional Intake TrackingundefinedDirections:undefinedRecord your meals, snacks, drinks, etc. over a 3 day period. For each meal, provide the date, your meal content, and determine the number of servings from each food group. Provide complete details and data (see example row below).undefined Date Record meal content here Record servings and of which food group Example: 5/12 Breakfast ½ cup of oatmeal (made with water), ¼ of raisins, 2 cups of coffee (with 1 tsp. sugar each). Grain: 1 oz. serving Fruit: ¼ cup dried fruit = ½ cup fresh fruit Sugar: 2 tsps. Nutritional Intake Analysis and Self-evaluationDirections:Review the Food Log of your intake for the 3 day period. In the chart below, for each day, record the number of servings you consumed from each category. Analyze your intake compared to the USDA guidelines and answer the self-evaluation questions in complete sentences, using proper spelling and grammar.undefined Dairy Servings Protein Servings Fruit Servings Vegetable Servings Grain Servings Sugar Servings Fat Servings Day 1 Day 2 Day 3 Daily USDA Dietary Guidelines for an Adult* 3 cups 5.5 ounces 2 cups 2.5 cups 6 ounces minimal minimal * Based on a 2000 calorie diet for an 18+yr old adult according to the 2015-2020 Dietary Guidelines.Self-evaluation Questions:In which areas of your nutritional intake did you do well?Which areas of your nutritional intake need adjustment?What are some specific eating/drinking changes you could make to better balance your nutritional intake?After analyzing your nutritional intake and comparing it to the USDA guidelines, what surprised you?
WEAX 201 ERAU Daytona Beach Meteorology Earths Atmosphere Discussion
Choose TWO questions - one from each question set below - to answer. Place your chosen questions and the formulated answer ...
WEAX 201 ERAU Daytona Beach Meteorology Earths Atmosphere Discussion
Choose TWO questions - one from each question set below - to answer. Place your chosen questions and the formulated answers* within a separate document that includes your name at the top.*Your answers will be scored on completeness, correctness, clarity, and conciseness, and the degree of synthesis and application of the related concepts, not on how many words they contain. More is not necessarily better!Question Set 1: Weather Applications in Everyday LifeDescribe the general trends in pressure and temperature data collected as a radiosonde attached to a weather balloon rises through the troposphere. Support your answer by synthesizing and applying concepts explored in Module 1 regarding the vertical structure of the atmosphere.During a summer vacation to Mammoth Mountain, California, you and some friends are planning to take the ski lift to the top of the mountain, at nearly 11,000 feet above sea level, and descend on bikes. Assuming there is no rain in the forecast, how should you and your friends prepare for this adventure based on the expected changes in atmospheric conditions during the descent? Support your answer by synthesizing and applying concepts explored in Module 1 regarding the vertical structure of the atmosphere.Assume that the weather at the Embry-Riddle campus in Daytona Beach, FL, is currently dominated by low pressure, and that the weather at the Embry-Riddle campus in Prescott, Arizona, is currently dominated by high pressure. Synthesize and apply concepts explored in Module 1 to describe the expected differences in the current weather conditions at each location based on the stated differences in relative pressure. Your discussion should include references to several specific weather elements.Synthesize and apply concepts explored in Module 1 to explain how you could determine the approximate locations of surface high and low-pressure systems by viewing a weather satellite image of cloud cover over the United States.Question Set 2: Weather Applications in AviationYou are a member of an airport development firm who has been contracted to build two new regional airports – one in a valley nestled in high mountains and one near an ocean coastline. Synthesize and apply concepts explored in Module 1 to explain why your firm should consult a climate expert in the planning process.Synthesize and apply concepts explored in Module 1 regarding the vertical structure of the atmosphere to explain the need for commercial aircraft cabin air pressurization.The upward lift on an aircraft wing is directly proportional to the atmospheric density. All other factors aside, will the upward lift on an aircraft wing increase or decrease as it gains altitude? Synthesize and apply concepts explored in Module 1 to justify your answer.After choosing any TWO weather elements described in Module 1, make specific connections between each weather element and expected or potential impacts to flight operations (during either takeoff, landing, or en route).https://www.weather.gov/jetstream/layershttps://scied.ucar.edu/learning-zone/atmosphere/we...https://www.weather.gov/jetstream/pressurehttps://www.youtube.com/watch?v=zz_CRzcIT-Qhttps://www.youtube.com/watch?v=zz_CRzcIT-Q
8 pages
Lab Report Meiosis
During the interphase stage, there is rapid synthesis of protein thus increased growth rate of the cell to almost double s ...
Lab Report Meiosis
During the interphase stage, there is rapid synthesis of protein thus increased growth rate of the cell to almost double size of the mother cell. The ...
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