Group Name: Anyone Else Incandescent light Lab Order Diptera Lepidoptera Coleoptera Thysanura Trichoptera Neuroptera # of Ind 81 13 6 2 16 3 Partial Lab Report 3 (Results and Discussion) Possible Sections Name, Section, Title, Date, Location, Physical conditions 0.25 Results Adequate narrative section with approriate references to tables, figures, etc. Appropriate tables and figures with proper legends Include T-test, similarity coefficient, and any other statistics that may be appropraite. 3.00 2.00 2.00 Discussion Adequate discussion of results Comparison with a published study (1) Restate thesis and predictions in relation to results 3.00 1.00 1.50 Overall Writing Correct spelling, grammar, sentence structure, word choice Correct reference format: AMA Format 1.00 1.25 TOTAL 15.00 ORDER Lepidoptera Diptera Coleoptera Thysanoptera Dermaptera Siphonaptera Trichoptera Odonata # Individuals 25 870 34 14 1 2 63 2 Blattodea Hemiptera 3 1 Hymenoptera Psocoptera Homoptera 26 3 4 Isoptera 2 Total 1050 ORDER # Individuals Diptera Coleoptra Orthoptera Hemiptera Homoptera 1158 42 6 7 2 Lepidoptera Thysanoptera Trichoptera Neuroptera 28 20 16 6 Blattaria Epidtera Dermaptera 2 89 2 Total 1378 Troy University Dept. of Biological and Environmental Sciences BIO L229 – GENERAL ECOLOGY LAB – Fall 2018 Light Sampling for Adult Invertebrates Several methodologies have been developed to sample for adult flying insects. This laboratory exercise will utilize on the light trapping method to collect data in two distinct areas under two different light sources. Light trapping is a simple sampling method that uses a translucent material of a known size and a central light source. Ecologists often utilize sampling techniques to gather information about a population or community in which it is difficult to take a census of all individuals in an area of interest. From the data collected, inferences can be made about the entire population or community. The data collected during this exercise can be used to compare species composition and distribution found in the two unique areas as well as assemblage affinity for light source. Objectives 1. To hone ability to create and test hypotheses. 2. To become familiar with common sampling methods for adult invertebrates. 3. To become familiar with identification of common flying invertebrates. Methodology 1. Choose an appropriate location for sampling in your designated area (area with two trees appx. 8ft apart) 2. Tie a rope between both trees about 6ft above the ground. 3. Hang battery powered light source with your designated light source type at the appx. Center of your rope. 4. Take a sheet of know size and drape it over the rope with your light source at the center of the sheet. 5. Pull corner of the sheet taught to an appx. 45-degree angle creating a tent-like shape. 6. At the base of each side place a container for collection in step 8. 7. Turn light source on at designated time and keep it illuminated for 45 minutes. 8. Upon completion of the 45-minute period, using a squirt bottle, spray 70% ethanol on the insects and scrape them into the container. 9. Fill the remainder of the container with 70% ethanol. 10. Sorting and identification will occur in the following lab. Introduction to Insect Behavior What will be required from this experiment.  Next week, Tuesday the 9th: Quiz  Following Tuesday the 16th: Partial Lab Report (Results and Discussion) What is behavior  Behavior is any action that an individual carries out in response to a stimulus or its environment, especially an action that can be observed and described. Common Behavior of Insects  Attraction to light  WHY??  Phototactic What does this have to do with what we are doing? Light Trapping  Common method for collection of adults insects  Exploits insects phototactic behaviors Treatments What will we find?  Lepidoptera  Trichoptera  Neuroptera  Diptera Lepidoptera  Butterflies and Moths  Likely only going to see moths Trichoptera  Caddisflies Neuroptera  Lacewings (net-winged insects) Diptera  True flies  Almost everything you consider to be a fly and more Set up  Along the trail, Find two trees appx 5’-8’ apart.  Tie a rope taught at appx 5’ off the ground  Hang a sheet over the rope  Position light source under the sheet Before set up Create a hypothesis and predictions  Develop a hypothesis within your group regarding the Density and Diversity between the two light treatments.
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