Bio lab report

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Science

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you can find the results tables and everything related to the lab in the attachments. Also you can find the Testing Hypothesis Worksheet too. 2 pages will be fine for the report but make sure to include everything such as:

    • Title (2 pts)
    • Methods Overall and Experimental Design (20 pts)
    • Results (8 pts)
    • Figures (12 pts)
    • Discussion Conclusions and Context/Significance (20 pts)
    • References (8 pts)--3 references required, at least 2 of which MUST be primary articles. The third can either be another primary or a "reliable" secondary article
    • Writing Style (10 pts)

    the type of mushrooms experimental in the lab are : Cermini - White button - Portabella

    For the testing hypothesis form will be 2 pages. 2 sentences will be enough for each box.

    For the references I need it to be in CSE 8th edition

    I don't need it to be perfect 100%

Unformatted Attachment Preview

Testing Hypotheses Worksheet Name: Date: Section: TA: CSE Citation: Research Question  If… Hypothesis (Proposed explanation or possible answer)  Experiment (Description of how the hypothesis is tested—independent variables, dependent variables, controls, and constants) And…  Then… Predicted Result (What is expected if the hypothesis is true)  Actual Result  Was the hypothesis supported (write “Yes” or “No” below)?:  Therefore… Conclusion  Future Directions, New Hypotheses, or Next Steps 5 Investigating Enzymes-Mushroom Metabolism Write your question and hypothesis below: Do Mesrooms that grow in tells and have similar levels of cell base If wehears grow in fields and a melho , and their levels of cellubiase are measured in an experiance, then the cellubiase levels will love shown to be at around the same levels Lab 6 | Investigating Enzymes-Mushroom Metabolism Write out your plans for your experiment below: 1 ) Wiegh out la gran reach of the three samples 2) loe a mortar and pesti ontill paste 3 Trades to micoentrifuge 4 collect the clear flid Incubation 5) Create solutions is the frid 6) create soutions w pure etyme y create standard curve iga bulion for va menetes 01 Foc absordane valies for created soktion 9) teyyare to standard une 10) compare values w/ other mushrooms betoi reaction mix is basic (high pH) and dena phenol to turn yellow. Setting up Standards Use the information in the following table to mix standards for constructing a standard curve. A stock solution of p-nitrophenol will be provided at a concentration of 200 nM. Use the CV, = C2V, calculation to determine how to dilute the stock solution with wa- ter to get the remaining known concentrations of standards. (Hint: V2 = the volume of p-nitrophenol + volume of water = 500 uL.) 1. Make dilutions of p-nitrophenol according to your calculations in the table below. Mix the stock with water for each standard solution directly in labeled cuvettes. 2. Add 500 uL of stop solution to each cuvette. 3. Follow the instructions for “Quantifying p-Nitrophenol” to set up the spectrophotome- ter and record absorbance values. Table 3 Standards for calculating p-nitrophenol concentration. Final Volume in Cuvette Absorbance at 410 nm p-Nitrophenol Concentration (nM) Volume of 200 nM p-Nitrophenol (PL) Stop Solution (UL) Volume Water (uL) (PL) 0.000 1,000 uL 500 uL 500 ul 0 Ο μL 500 ul 62.5-1437.31 1,000 ML 25 nM 0.226 50 nM 125 L 375 L 500 ML 1,000 ML 0.410 100 nM 250 L 250L 500 ML 1,000 uL 0.837 200 nM (provided 5os h 500 ML stock solution) 1.543 1,000 ul to test tubes 2 ㅋ cuvettes with the stop solution. p-nitrophe- reactions, pipette 500 uL of stop Note: If you are using short timepoints, pipette the stop solution prior to setting up the reaction. 4. Prepare a "blank.” Mix 62.5 uL the supernatant from the mushroom extract (containing cellobiase enzyme) with 750 uL of 0 mM substrate solution (containing no nyl glucopyranoside) in a 1.5-mL microcentrifuge tube. 5. Follow the instructions for "Quantifying p-Nitrophenol” below to set up ples to the standard curve to determine p-nitrophenol concentration as an indication spectro- the Table 2 Absorbance at 410 nm Sample Volume of Reaction Mix (Mushroom Extract + Substrate) (PL) Volume of Stop Solution (HL) Final Volume in Cuvette (PL) 0,901 500 ML 500 ML 1,000 ML creminine White button Portabella 500 uL 500 uL 1,000 ML 0.543 1.102 د 500 uL 500 uL 1,000 uL 500 uL 500 uL 1,000 ML 500 uL 500 uL 1,000 IL ontrolling Cellobiase Reaction Times zymatic reactions are often time sensitive. Typically, the more time the reaction has n, the more product you'll get. Depending on your question, you may want to measu reactions after they run the same amount of time (time would be a constant), or y y want to measure at multiple time points (if you are interested in comparing rates ymatic activity over time). Cellobiase reactions will produce measureable produ in minutes. As you design your experiment, pick a reaction time around 15 minu
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