Design an experiment using SDS-PAGE to determine the molecular size of the protein 4PN6

timer Asked: Nov 21st, 2018

Question description

I have completed part A, will send the file to the writer after I confirm.

B)Design an experiment using SDS-PAGE to determine the molecular size of the protein.

a.Present your experiment as a flow chart and indicate the rationale behind each step besides the flow chart.

b.Draw a gel diagram to indicate the expected size of the protein.

Some guiding questions:

1)Are there any inter/intra molecular disulphide bonds in the protein?

2)Are there any other post-translational modification to the protein that may affect the size of the protein?

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