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Need help to answer these questions

About clinical chemistry please note this is clinical chemistry not general chemistry.

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Assignment Explain the 13 components of integrated and modular systems Assignment 1 • Twenty thousand patients are tested last year for Lyme diseases using a new assay and 12,000 tested positive. Of these 12000 that tested positive, 600 did not have Lyme disease. Of the 8,000 that tested negative, 40 actually had Lyme disease. Find the sensitivity, the specificity, the PPV and the NPV. Assignment 2 • What is the positive predictive value of a test in which 220 tested individuals with positive test results actually have the disease and 45 tested individuals with positive test results did not have the disease? Assignment 3 • One of your classmates has invented a new kit to diagnose for this year seasonal flu. He is validating his new kit by testing six hundred patients who all showed symptoms of having a seasonal flu. From those 425 tested positive with the new kit and of these 425 patients, 25 were verified by culture to actually be negative. Of the patients who tested negative, 10 actually had flu. Find the diagnostic sensitivity and specificity for this new flu test kit. Example #2 A solution of Co(H20)2+ has an absorbance of 0.20 at 530 nm in a 1.00 cm cell. E is known to be 10 L·mol-1.cm-1. What is its concentration. Example #3 The absorbance of an unknown MnO solution is 0.500 at 525 nm. When measured under identical conditions, a 1.0x104 M MnO is found to have an absorbance of 0.200. Determine the concentration of the unknown. Assignments Cakulate the absorbance of a solution having a % of 89 at 400 nm. Assignments The percent transmittance of a solution is 50%. Calculate the absorbance. Reading Assignments (Bonus mark) Explain the principle of Atomic absorption Spectrometry Fluorometry Nephelometry, Turbidimetry and ,C. Review Questions of electrophoresis:- aasing 1 1. What is electrophoresis? State the underlying principle .2 How does each of the following factors affect.3 the migration rate of a substance being ?electrophoresed ... Ionic strength of buffer ,b. buffer pH electrical field ... charge of molecule What is the principle of protein .4 electrophoresis? What specific characteristics of proteins are important in their ability to ?migrate What are the differences and similarities .5 ?between electrophoretic techniques What other clinically relevant substances.6 besides albumin and globulins are examined ?using electrophoretic techniques Make a list of interferences that can affect 3 protein electrophoresis and how these are .mitigated What is capillary electrophoresis and what is its. clinical utility in the chemistry laboratory? Make a list of specific analytical assays (including nucleic acid testing) that rely on .capillary electrophoresis What specific physiological disorders can 9 ?electrophoretic techniques distinguish
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