Heat Microbiology

Jun 27th, 2015
Studypool Tutor
University of California - Riverside
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Description of heat microbiology includes hanging drop, wet mount preparations, simple stains, gram stains, pure bacterial colonies, pour plates and streaking techniques,

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Exercise 1: Moist and dry heat1. How are microorganisms destroyed by moist heat? By dry heat?Microorganisms are destroyed by moist heat by denaturing the proteins in the microorganism. Denaturation mean a change in the physical or chemical property of a protein. It also includes the breaking of chemical bonds holding the three dimensional protein. The dry heat act like an oven that changes the microbial proteins by oxidizing the protein molecules and creating an arid internal environment. Dry heat killed the microorganisms by causing damage and oxidizing protein molecules from the microorganisms. Most dry heat destroys microorganism by also denaturing proteins. 2. Are some microorganisms more resistant to heat than others? Why?Yes, some microorganisms are more resistant to heat than others because of their genetic and biological makeup. These microorganisms are called thermophile, which can live at relatively high temperature, between 45 and 122C. Thermophile can survive at high heat because of their enzymes, which can withstand and function high temperatures. These heat stable enzymes, such as DNA polymerases are used in molecular biology today. An example is PCR. The bacteria are more heat resistant because their membrane and proteins are stable at these extremely high temperatures. 3. Is moist heat more effective than dry heat? Why?Moist heat is more effective than dry heat because moist heat can penetrates materials much more faster tha

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