BIOCHEMICAL SEPARATIONS

Jun 29th, 2015
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This course will consider the recovery of proteins from dilute solutions by partition from a liquid to a solid (adsorbent) phase. Many of the concepts described will also apply to the recovery and separation of smaller molecules which is covered in more detail in the Chemical Separations module.

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BIOCHEMICAL SEPARATIONS Adsorption and Chromatographic separationsDr John HubbleIntroductionThis course will consider the recovery of proteins from dilute solutions by partition from a liquid to a solid (adsorbent) phase. Many of the concepts described will also apply to the recovery and separation of smaller molecules which is covered in more detail in the Chemical Separations module. The course will start by considering the types of partition phenomena which can be employed in protein separations. This will lead on to a quantitative assessment of adsorption where loading and elution are operated as sequential process steps and will include descriptions of batch separations in stirred tanks and packed bed adsorption. Finally, chromatographic separations will be discussed where the partition conditions cause adsorption/desorption to occur simultaneously such that materials progress through a packed bed at different rates.It should be stressed that the complexity of biochemical separations dictates a largely empirical approach. Design equations serve a valuable role in the design of pilot scale evaluations, but cannot, as yet, be expected to fully describe the performance of a industrial separation. BackgroundBasis of separationMost protein recovery processes will require that a specific protein product be selectively removed and concentrated from a complex mixture usually containing a number of unwanted proteinaceous by products. Therefore techniq

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