Differentiation of Staphylococcus

Jun 29th, 2015
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University of California - Riverside
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Laboratory #3Differentiation of StaphylococcusSkills= 20 PointsObjective:At the completion of this activity, the student will be able to:1.Correctly differentiate Staphylococcus aureus, Staphylococcus epidermidis and Staphylococcus saprophyticus through the use of colony morphology, catalase test, hemolytic properties, gram stain, coagulase tests, Staphyloslide and Novobiocin sensitivity.Materials:Blood agar cultures of:Staphylococcus aureusStaphylococcus epidermidisStaphylococcus saprophyticus3% hydrogen peroxide (Catalase reagent)2-Microscope slides MicroscopeImmersion oilCoagulase plasmaGram stain materials1-Staphyloslide2-Novobiocin disks1-TSA References:1. Mahon and Manuselis, Textbook of Diagnostic Microbiology, Fourth Edition, Chapter 142. BD BBL Coagulase Plasma package insert3. BD BBL Staphyloslide package insertPrinciples:Bacteria in the genus Staphylococcus are gram positive spherical cells that occur singly, occasionally in pairs, but most frequently in irregular clumps. The appearance of a gram stained smear is usually sufficient to distinguish staphylococci from the streptococci because of the characteristic cell grouping (grape-like clusters) of staphylococci. These two groups may also be distinguished by the presence of the enzyme catalase, S. aureus being catalase positive and Streptococcus catalase negative.Staphylococci are normal flora on the skin and mucous membranes, but

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