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J. Agric. Sci. Technol. (2008) Vol. 10: 493-499RESEARCH NOTESIsolation, Identification and Differentiation ofLocal B. thuringiensis StrainsM. Keshavarzi1ABSTRACTIn this research, 514 soil samples and dead larvae were collected from Khorassan,Lorestan, Tehran, Ghazvin, East Azarbaijan, West Azarbaijan, Mazandaran andHamedan Provinces. B. thuringiensis was isolated from the samples using a heat-acetatemethod and the isolates were identified and classified using biochemical tests. Thefrequency of B. thuringiensis in soils with different plant communities was studied.According to results, 127 isolates were collected from the samples collected. Most isolatesproduced atypical and heterogenic and some bipyramidal crystals. Nearly all the isolateswere able to hydrolyze starch and gelatin and ferment glucose and fructose, but could notproduce indole and H2S or ferment galactose and lactose. The isolates were divided into 8biochemical types, among which B. thuringiensis subsp. kurstaki was the most frequenttype. Total Bt frequency, corresponding to the whole sampling areas, was calculated asbeing 3.1%; the highest frequency was recorded for Khorassan Province (5.1%) and thelowest for Lorestan Province (0%). No realtionship was found between B. thuringiensisfrequency and vegetation status of the soils examined.Keywords: B. thuringiensis, Biochemical type, Isolation.B. thuringiensis strains, in any particularcollection may be characterized in a numberof ways su

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