Fractionation of lipids and purification of c-linolenic acid (GLA) from Spirulina platensis

Jun 30th, 2015
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The blue-green alga, Spirulina platensis, is a potential source of GLA, an essential polyunsaturated fatty acid of excellent economic interest. GLA is a metabolite of linoleic acid (LA) and the first intermediate in the conversion of LA to arachidonic acid (AA) (Gunstone, 1992). Various studies have asserted the pharmaceutical value of GLA, especially in lowering the low density lipoprotein in hypercholesterolemic.

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Available online at www.sciencedirect.comFood Chemistry 109 (2008) 580586www.elsevier.com/locate/foodchemFractionation of lipids and purication of c-linolenicacid (GLA) from Spirulina platensisM.G. Sajilata, R.S. Singhal *, M.Y. KamatFood Engineering and Technology Department, Institute of Chemical Technology, University of Mumbai, Mumbai 19, IndiaReceived 1 July 2007; received in revised form 24 December 2007; accepted 10 January 2008AbstractThe microalgae, Spirulina platensis, is an excellent source of c-linolenic acid (GLA), an essential polyunsaturated fatty acid and apotent nutraceutical. The fatty acid composition of S. platensis ARM 740 was determined after transmethylation by gas chromatography(GC). Lipid fractionation was achieved on silica gel column chromatography and preparative TLC. Neutral lipids, glycolipids and phospholipids accounted for 77.0%, 15.6% and 7.4%, respectively, of the total lipid fraction. S. platensis ARM 740 was found to contain 94%of the total GLA in the glycolipid fraction. Attempts were made to purify GLA methyl ester by using urea to form inclusion complexeswith the saturated and the less unsaturated FAMEs (fatty acid methyl esters), which enhanced the purity of GLA methyl ester to 84%. Afurther approach to isolate GLA methyl ester with higher purity involved the use of argentated silica gel chromatography. An initialPUFA concentration step frequently adopted by most researchers to increase GLA purity was not necessa

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