Isolation and characterization of acidophilic bacterium from Gaofeng Mine in China

Jun 30th, 2015
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Highly acidic environment formed by the oxidation of pyrite and other sulfidic minerals is known to be populated by a range of acidophilic and acid-tolerant prokaryotic and eukaryotic life forms. The most studied is Acidithiobacillus ferroxidans. A. ferrooxidans is an acidiophilic chemolithotrophic gramnegative bacteria, which can derive energy from the oxidation.

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Isolation and characterization of acidophilic bacterium fromGaofeng Mine in ChinaYANG Yu( )1, 2, QIAN Lin( )1, SHI Wu-yang()1,PENG Hong( )1, QIU Guan-zhou()1, 21. School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China,2. Key Laboratory of Biometallurgy of Ministry of Education, Central South University,Changsha 410083, ChinaReceived 28 September 2007; accepted 10 December 2007Abstract: An acidophilic, chemolithotrophic and ferrous oxidizing bacterium strain GF was isolated from the acid mine drainage(AMD) of Gaofeng Mine, Guangxi Province, China using 9K enrichment medium, and then purified on solid ferrous-agarosemedium. The physiological experiments show that it can use ferrous or sulfur as sole energy and a low level (0.1%, w/v) of peptonecan accelerate the growth of the isolated strain. The optimum pH and temperature for growth are 2.0 and 30 , respectively. Theisolated strain shares 99.64% identities of 16S rRNA gene with the type strain Acidthiobacillus ferrooxidans ATCC 23270 and 100%identities of iro gene (CDS) with A. ferrooxidans strain Fe-1. These results show that the strain can be considered as Acidthiobacillusferrooxidans. Because of the high activity of oxidizing ferrous and sulfide mineral, strain GF was used in bioleaching of marmatite.The Zn concentration is 0.273 g/L under the steriled control and 7.30 g/L with adapted GF strain incubated after 29 d in leac

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