Pathology notes

Apr 16th, 2015
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LEUKEMIASWORKUP/INVESTIGATIONBYH12/70275/11Bbitok thomasInvolves 1.labaratory studies 2.imaging 3.histologic findings1.Laboratory studiesCBC and differentialsBone marrow aspirationImmunophenotypingHistochemical stainsChromosomal analysisi)CBC and differentials some abnormalities in CBC AML-blasts are present in peripheral smear CLL lymphocytosis must be greater than 5000/mm3 Absolute neutrophil count-normal RBC and platelet count-mildly decreased Peripheral smear or bone marrow-normal maturesmall lymphocytes with <50%blast forms CMLdefined by its peripheral blood countLeukocytosis >100000/mm3Diff count-neutrophil precursors present-basophilia-easinophilia NB mature andfunctional unlike AMLii)Bone marrow aspiration Establish dx of leukemia Morphology of blast- diff ALL&AML ALLHomogenous infiltrate of lymphoblastsreplace BM elementsLymphoblasts- small, 14 um diameter, scantcytoplasm, nucleus-no nucleolus or small one AML 30% nucleated cells-blasts of myeloid origin Myeloblasts-multiple nucleoli,delicate chromatin,grayblue cytoplasm, auer rods (virtualy dx of AML, no otherleukemia) CLL BM infiltrate >30% lymphocytes( mature, <55% blastforms) Nuclei round, cytoplasm scant,chromatin compact,nucleoli inconspicous, mitotic figures rareiii) ImmunophenotypingUsing multiparameter flow cytometery following label

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