Pathology notes

Apr 16th, 2015
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STAINS USED IN HAEMATOLOGYBy NAFULA NYONGESAStaining blood and bone marrow films Romanowsky stains are universally employedbecause of the satisfactory results. They make subtle distinctions in shades ofstaining and of staining granules differently Staining depends on 2 components; Azure B(trimethylthionin) Eosin Y(tetrabromofluorescein)Romanowsky stains now in use Jenners Giemsas LeishmansN/B:The results are inferior compared tocombined stains ieMay-Grunwald-GiemsaJenner-GiemsaAzure B-Eosin y methodsStaining principle Mechanism of staining depends on complexdifferences in binding of the dyes to chemicalstructures and interactions between the dyemoleculesColour response to Romanowsky Acidic groupings of nucleic acids , proteins ofnuclei and primitive cytoplasm determinetheir uptake of the basic dye(azure B) Presence of basic groupings on haemoglobinmolecules results in its affirnity for acidic dyesand its staining by eosin Granules in the cytoplasm of neutrophil areweakly stained by azure complexesCont. Eosinophilic granules contain a sperminederivative with an alkaline grouping whichstains strongly with acidic component of dye Basophilic granules contain heparin which hasan affirnity for the basic component of the dye The effects depend on molar equilibrium betweenthe two dyes In time dependant reactionsAutomated staining Using machines n handles large ba

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