Pathology notes

Apr 16th, 2015
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ElectrophoresisPresentation by Caesar OkatchDefinitionMigration of charged particles inan electric field.Electrophoretic techniques inclinical laboratoriesMoving boundary electrophoresisZone electrophoresisCapillary zone electrophoresisMoving boundary electrophoresis Developed in the 30s by Arne Tiselius He used a u-shaped electrophoretic cell and used aSchlieren band optical system to detect the degree ofrefraction by the proteins as they passed through themedium So, he mixed a protein solution with a buffer and layeredthe solution underneath the same buffer. The buffer was then connected to electrodesZone electrophoresis Uses a solid support medium that is then stained toenable observation and analysis Eliminated the problem of the indiscrimination of subtledifferences that was brought by the Schlieren opticalsystem of analysis The support mediums that were used include; the filter paper; required several hours(overnight), poorresolution, opacity which was frustrating densitometry Agar: contains agarose and agaropectin Cellulose acetate: simplicity, reproducibility, ease ofdensitometry, low costCapillary Zone Electrophoresis(CZE) A small volume sample is aspirated into a thin fused silica capillarytube. The negative charge on the interior of the tube and its relative smalldiameter leads to a strong net negative charged surface area. Under conditions of electrophoresis, this set

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