Pathology notes

Apr 16th, 2015
Studypool Tutor
Price: $5 USD

Tutor description

Word Count: 1032
Showing Page: 1/32
ElectrophoresisPresentation by Caesar OkatchDefinitionMigration of charged particles inan electric field.Electrophoretic techniques inclinical laboratoriesMoving boundary electrophoresisZone electrophoresisCapillary zone electrophoresisMoving boundary electrophoresis Developed in the 30s by Arne Tiselius He used a u-shaped electrophoretic cell and used aSchlieren band optical system to detect the degree ofrefraction by the proteins as they passed through themedium So, he mixed a protein solution with a buffer and layeredthe solution underneath the same buffer. The buffer was then connected to electrodesZone electrophoresis Uses a solid support medium that is then stained toenable observation and analysis Eliminated the problem of the indiscrimination of subtledifferences that was brought by the Schlieren opticalsystem of analysis The support mediums that were used include; the filter paper; required several hours(overnight), poorresolution, opacity which was frustrating densitometry Agar: contains agarose and agaropectin Cellulose acetate: simplicity, reproducibility, ease ofdensitometry, low costCapillary Zone Electrophoresis(CZE) A small volume sample is aspirated into a thin fused silica capillarytube. The negative charge on the interior of the tube and its relative smalldiameter leads to a strong net negative charged surface area. Under conditions of electrophoresis, this set

Review from student

Studypool Student
" Excellent work as usual "
Ask your homework questions. Receive quality answers!

Type your question here (or upload an image)

1821 tutors are online

Other Documents


Brown University

1271 Tutors

California Institute of Technology

2131 Tutors

Carnegie Mellon University

982 Tutors

Columbia University

1256 Tutors

Dartmouth University

2113 Tutors

Emory University

2279 Tutors

Harvard University

599 Tutors

Massachusetts Institute of Technology

2319 Tutors

New York University

1645 Tutors

Notre Dam University

1911 Tutors

Oklahoma University

2122 Tutors

Pennsylvania State University

932 Tutors

Princeton University

1211 Tutors

Stanford University

983 Tutors

University of California

1282 Tutors

Oxford University

123 Tutors

Yale University

2325 Tutors