university of florida bsc2010 biology 1 ch20 slides

May 6th, 2015
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university of florida bsc2010 biology 1 ch20 slides

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Fig. 20-2Bacterium1 Gene inserted intoplasmidBacterialchromosomeCell containing geneof interestPlasmidRecombinantDNA ( plasmid )Gene ofinterestDNA ofchromosome2 Plasmid put intobacterial cellRecombinantbacterium3 Host cell grown in cultureto form a clone of cellscontaining the clonedgene of interestGene ofInterestProtein expressedby gene of interestCopies of geneBasicProtein harvested4 Basic research andvarious applicationsresearchon geneGene for pestresistance insertedinto plantsGene used to alterbacteria for cleaningup toxic wasteProtein dissolvesblood clots in heartattack therapyBasicresearchon proteinHuman growth hormone treats stuntedgrowthFig. 20-3-3Restriction siteDNA15335Restriction enzymecuts sugar-phosphatebackbones.Sticky end2DNA fragment addedfrom another moleculecut by same enzyme.Base pairing occurs.One possible combination3DNA ligaseseals strands.Recombinant DNA moleculeFig. 20-4-4HummingbirdcellTECHNIQUEBacterial cellamp R geneBacterialplasmidlacZ geneRestrictionsiteStickyendsGene of interestHummingbirdDNA fragmentsNonrecombinantplasmidRecombinant plasmidsBacteria carryingplasmidsRESULTSColony carrying nonrecombinant plasmidwith intact lacZ geneColony carrying recombinantplasmid with disrupted lacZ geneOne of manybacterialclonesFig. 20-5Foreign genomecut up withrestrictionenzymeLarge plasmidLa

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