university of florida bsc2010 biology 1 ch21 slides

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university of florida bsc2010 biology 1 ch21 slides

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Fig. 21-1Fig. 21-2-2ChromosomebandsCytogenetic mapGenes locatedby FISH1Linkage mappingGeneticmarkersFig. 21-2-3ChromosomebandsCytogenetic mapGenes locatedby FISH1Linkage mappingGeneticmarkers2Physical mappingOverlappingfragmentsFig. 21-2-4ChromosomebandsCytogenetic mapGenes locatedby FISH1Linkage mappingGeneticmarkers2Physical mappingOverlappingfragments3DNA sequencingFig. 21-3-11 Cut the DNAinto overlappingfragments short enoughfor sequencing2 Clone the fragmentsin plasmid or phagevectors.Fig. 21-3-21 Cut the DNAinto overlappingfragments short enoughfor sequencing2 Clone the fragmentsin plasmid or phagevectors.3 Sequence eachfragment.Fig. 21-3-31 Cut the DNAinto overlappingfragments short enoughfor sequencing2 Clone the fragmentsin plasmid or phagevectors.3 Sequence eachfragment.4 Order thesequences intoone overallsequencewith computersoftware.Fig. 21-4Table 21-1Fig. 21-7Exons (regions of genes coding for proteinor giving rise to rRNA or tRNA) (1.5%)RepetitiveDNA thatincludestransposableelementsand relatedsequences(44%)L1sequences(17%)Introns andregulatorysequences(24%)UniquenoncodingDNA (15%)RepetitiveDNAunrelated totransposableelements(15%)Alu elements(10%)Simple sequence Large-segmentDNA (3%)duplications (56%)Genomes of humans, rats, andmice show high sequenceconservation for about 500 noncoding regions.

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