university of florida bsc2010 biology 1 ch18 19 slides

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university of florida bsc2010 biology 1 ch18 19 slides

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BSC2010 LectureChs. 18-20Fig. 18-2PrecursorFeedbackinhibitiontrpE geneEnzyme 1trpD geneRegulationof geneexpressionEnzyme 2trpC genetrpB geneEnzyme 3trpA geneTryptophan(a) Regulation of enzymeactivity(b) Regulation of enzymeproductionFig. 18-3trp operonPromoterPromoterDNAtrpRRegulatorygenemRNA5ProteinGenes of operontrpE3OperatorStart codonmRNA 5RNApolymeraseInactiverepressorEtrpDtrpBtrpABAStop codonDCPolypeptide subunits that make upenzymes for tryptophan synthesis(a) Tryptophan absent, repressor inactive, operon onDNANo RNA mademRNAProteintrpCActiverepressorTryptophan(corepressor)(b) Tryptophan present, repressor active, operon offFig. 18-4RegulatorygenePromoterOperatorlacZlacIDNANoRNAmade3mRNARNApolymerase5ActiverepressorProtein(a) Lactose absent, repressor active, operon offlac operonDNAlacZlacY-GalactosidasePermeaselacI3mRNA5RNApolymerasemRNA 5ProteinAllolactose(inducer)lacAInactiverepressor(b) Lactose present, repressor inactive, operon onTransacetylaseFig. 18-5PromoterOperatorDNAlacIlacZRNApolymerasebinds andtranscribesCAP-binding siteActiveCAPcAMPInactiveCAPInactive lacrepressorAllolactose(a) Lactose present, glucose scarce (cAMP levelhigh): abundant lac mRNA synthesizedPromoterDNAlacICAP-binding siteInactiveCAPOperatorlacZRNApolymerase

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