Enzyme Lab Instructions

Jul 8th, 2016
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Northwest Vista College
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Northwest Vista College, Biology 1406 LaboratoryEnzymesV21 of 6Name:Title: EnzymesPre-Lab Questions1. Enzymes are what type of macromolecule?2. In general, what is the purpose of enzymes?3. What is the name of the process by which enzymes lose their 3-dimensional shape? What happens to theirfunction when this occurs? What are two factors that might cause this to occur?4. What is a positive control? What is a negative control? Why are they both needed in any experiment?5. On a graph, which axis has the independent variable? Which axis has the dependent variable?Northwest Vista College, Biology 1406 LaboratoryEnzymesV22 of 6IntroductionEnzymes are proteins that catalyze (speed up) chemical reactions. Most enzymes end with -ase, for example, aprotease is an enzyme that works on proteins, so they are usually easy to recognize. The molecule with whichenzymes interact is called a substrate. A substrate is changed to a product after an enzyme reacts with it. Thepart of the enzyme that actually is in contact with the substrate is called the active site. Enzymes are very specificfor a particular substrate. For this reason, an organism such as a human has millions of enzymes. Each enzymecatalyzes one reaction and all these enzymes are necessary, so that life can be sustained.In the Figure 1, the larger shape represents an enzyme while the smaller shapes are all substrates.Figure 1. Modeling the shape of enzymes and their substrates.Question

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