Molecular genetics

Jul 12th, 2016
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For the double stranded DNA, which of the following base ratios always equals 1. Circle the correct answer/s. 2. In Meselson and Stahl's experiment, what kind of DNA molecules would be found after four replication cycles? 3. In the experiment by Meselson and Stahl using heavy nitrogen (15N) and E. coli DNA, the Zero (initial) generation had completely labeled DNA. If DNA replication is semi-conservative predict the proportion of heavy strands to light strands in the (a) third generation and (b) sixth generation if all replication rounds occur in 14N (unlabeled media). 4. What experiment demonstrated that the 5'-to-3' exonuclease activity of DNA polymerase I was essential for cell viability? ..... 19. The Thymine (T) of DNA is located at the 3’ end and Guanine of the same strand of the DNA is located at the 5’ end.

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Surname 1Name:Course:Professor's Name:Date:Molecular genetics answersQuestion 1. a. (A + T)/ (G + C)Question 2. B) Only intermediate DNA (15N-14N)Question 3.(a)(b)One band of heavy DNAThree bands of heavy DNAOne band of light DNAsThree bands of light DNAQuestion 4.In E. coli, the DNA Pol I polAex1 mutant pressure endures at 37C but dies at 42C. This wasexposed to be for the reason that the mutant DNA Pol I enzyme had common action at 37C butan out of order 5'-to-3' activity at 42C. This verified that the 5'-to-3' exonuclease action of DNAPol I was vital for cell continued existence.Question 5.Both start replications as a spherical fragment, and in E. coli, the maternal DNA strands continuein a spherical formula through the repetition cycle. A repetition effervesce unlocks to form twoduplication junctions, and repetition goes on in a bidirectional. Lambda phage DNA is replicatedby a progressing loop appliance, in which a cut is made in one of the two strands of the circle,and the 5' end of the cut DNA strand is revolved out as a free pangs of increasing dimension asSurname 2repetition proceeds. The maternal DNA disc is replicated unceasingly, while the undeviatingbanished thread is replicated intermittently. As stretched as the loop lasts to roll, concatamers ofphage DNA can be produced. This later is cut up into linear chromosomes and packed into newphage crowns.Question 6.The foremost action was that they nurtured E. col

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