Dissertation results and discussion; Lab report

Jul 27th, 2016
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Dissertation results and discussion; Lab report; The test of reviewing the localization of the Hela cell Golgi apparatus within the cell cultures, the main aim of making the resident proteins visible is achieved consider (fig. 1, A-D). The observations under the fluorescent microscope and with the use of WGA with the buffer BSA or the NDS revealed a single antibody 25/15 homolog each in mammals. These resident proteins majorly share a conserved membrane topology and dilysine COPI binding motif. DISCUSSION studies thus back the postulation that antibody 25/15 works well in the early secretory pathway of the mammalian cells. When compared with other given integral membrane proteins that function in these particular transport steps, antibody 25/15 shows a similar dissemination pattern as observed for the different receptors and the 488 goat anti rabbit proteins.

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Running Head: DISSERTATION RESULTS AND DISCUSSIONDissertation results and discussionStudents NameUniversity Affiliation1DISSERTATION RESULTS AND DISCUSSION2AbstractIn this particular test of reviewing the localization of the Hela cell Golgi apparatus within the cellcultures, the main aim of making the resident proteins visible is achieved consider (fig. 1, A-D).The presence or lack of a specific antigen is clearly noted by the given pictures taken during thelab procedure and their observations confirmed by the fluorescent microscope of lens with givenmagnifications, for instance a lens with a magnification of 20 x. These results are indicated in(fig. 1 A-D). The antigen of use in this test is majorly the antigen-specific primary antibodiesextremely characterized with a probe or the fluorescent marker (ALBERTS, 2008). Theparticular antibody enabled the viewing of the protein sandwiched beneath the powerfulfluorescent microscope. Similarly, the secondary antibody with its conjugated fluorphore alsoenhanced clear viewing of the sandwiched proteins (Mas, 1996). As for the results that indicatedimmunopositive signals, ICC method enhanced the test. It also made it easier in thedetermination of cellular compartments that had the specific antigen of inquiry.IntroductionExperiments with the normal donkey serum (NDS) which mainly aids in the obstruction of thecross reactivity occurring in the background wind immunoglobulin present within themammalian ti

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