UV vis and Raman spectroscopy techniques

Aug 13th, 2016
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Harvard University
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characterization techniques explained in detail with example

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UV-VIS AND RAMAN SPECTROSCOPY:PRINCIPLE AND APPLICATIONS INNANOTECHNOLOGYPRESENTED TO:PROF. MANGALA JOSHIPRESENTED BY:MANALI SOMANI (2015TTF2390)SNEHA NAWAGE (2015TTF2395)ANUBHAV SHUKLA (2015TTFWhat is Spectroscopy? The study of molecular structure and dynamics through the absorption,emission and scattering of lightPrinciple of Spectroscopy The principle is based on is the measurement of the spectrum of samplecontaining atom/molecule Spectrum is a graph of intensity of absorbed or emitted radiation bysample verses frequency or wavelength Spectrophotometer is an instrument design to measure the spectrum of acompound.RAMAN SPECTROSCOPYConsider the collision between a photon and a molecule0sRaman Effect Scattering can be: Elastic- Scattered photon have the same energy andfrequency as the incident photons; it is called asRayleigh scattering Inelastic- A small fraction of light [approx. 1 in 107] isscattered at optical frequencies different than thefrequency of incident photons. This process ofscattering is termed the Raman effect Stokes Raman Scattering: Emitted photon is oflower frequency than incident photon Anti-stokes Raman Scattering: Emittedphoton is of higher frequency than incidentphotonInteraction between electric field of incident photon and molecule Light, with incident frequency n0, has an oscillating electric field (E) :E = E0 cos (2pn0t) Induces molecular electric

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