Introduction to the biology

May 20th, 2015
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1. The life on the earth formed before 3.5 billion years [earth has formed before 4.5 billion years] 2. Consisted of crust, mantel and core 3. The ancient atmosphere is with CO2,H2S, NH2, CH4,N2,H2

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Explain the following techniques1) RFLPRestriction Fragment Length Polymorphism (RFLP)is a difference in homologous DNA sequences that can be detected by the presence of fragments of different lengths after digestion of the DNA samples in question with specificrestriction endonucleases. RFLPs can be used in paternity cases or criminal cases to determine the source of a DNA sample. RFLPs can be used determine the disease status of an individual.ProcedureThe restriction enzyme is added to the DNA being analyzed and incubated for several hours, allowing the restriction enzyme to cut at its recognition sites. The DNA is then run through a gel, which separates the DNA fragments according to size. The separated fragments are thentransferred to a membrane via theSouthern blot procedure.2) RAPD It stands forrandom amplification of polymorphic DNA. It is a type of PCRrea

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