qPCR Lab Report and Analysis

Feb 3rd, 2012
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Boston University
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The qPCR procedure involves diluting control template andlibraries for quantificationto pM range. This involves adding 998 μl of the 0.1% Tween 20 to 2 μl of the unknown library template to make a 500-fold dilution. This will give an approximate concentration of 20 pM. Vortex the dilution to thoroughly mix the samples. Repeat steps 1 and 2 to produce 3 independent dilutions of the library template. Triplicate results for qPCR are important for subsequent analysis.

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